Expression Patterns of Collagen Types I and III in the Capsule of a Rat Knee Contracture Model
Expression Patterns of Collagen Types I and III in the Capsule of a Rat Knee Contracture Model
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DOI：
10.1002/jor.20997
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发表时间：
2010-03-01
影响因子：
2.8
通讯作者：
Itoi, Eiji
中科院分区：
文献类型：
--
作者：
Hagiwara, Yoshihiro;Ando, Akira;Itoi, Eiji
我们的目的是确定大鼠膝关节挛缩模型胶囊中I型和III型胶原蛋白的表达变化。将成年雄性大鼠单侧膝关节用刚性塑料板和螺钉固定于150度屈曲处，固定3天、1、2、4、8和16周（固定组）。假手术动物在股骨和胫骨上钻孔，并在没有钢板的情况下插入螺钉（对照组）。胶原链反应（qPCR）、免疫组化（IHC）和免疫印迹（WB）的表达模式。通过ISH和qPCR检测，与对照组相比，固定化后I型和III型胶原的表达减少。与对照组相比，IHC和WB固定后表达无变化。固定化后I型和III型胶原mRNA和蛋白的表达水平均未升高，说明这两种胶原的积累并非关节挛缩的病因。另一个过程，如囊和滑膜粘连，可能是关节挛缩的一个可能原因。(C) 2009骨科研究进展与进展[J] .中华骨科杂志，28 (3):331 - 331,2010
Our objective was to determine the changes in expression of collagen types I and III in the capsule of rat knee contracturde model. The unilageral knee joints of adult male rats were ragidly immobilized at 150 degrees of flexion using a rigid plastic plate and screws for 3 days, 1,2,4,8 and 16 weeks (immobilized group). Sham-operated animals had holes drilled in the femur and tibia with screws inserted without a plate (control group). The expression patterns of collagen chain reaction (qPCR), immunohistochemistry (IHC), and Western blotting (WB). Expressions of collagen types I and III were decreased after immobilization compared to the control group by ISH and qPCR. The expression was not changed after immobilized compared to the control group by IHC and WB. The expression of mRNA and protein levels of collagen types I and III were not increased after immobilization, which indicated that accumulation of the two types of collagen was not the etiology of joint contracture. Another process, such as capsule and synovial adhesions, may be one possible cause of joint contracture. (C) 2009 Orthopaedic Research Soceity Published by Wiley Periodicals, Inc J Orthop Res 28 315-321, 2010