Simultaneous quantitative determination of bupropion and its three major metabolites in human umbilical cord plasma and placental tissue using high-performance liquid chromatography-tandem mass spectrometry.
Simultaneous quantitative determination of bupropion and its three major metabolites in human umbilical cord plasma and placental tissue using high-performance liquid chromatography-tandem mass spectrometry.
复制标题
高效液相色谱-串联质谱法同时定量测定人脐带血浆和胎盘组织中的安非他酮及其三种主要代谢物。
DOI：
10.1016/j.jpba.2012.05.008
复制
发表时间：
2012
影响因子：
3.4
通讯作者：
Nanovskaya,TatianaN
中科院分区：
文献类型：
--
作者：
Wang,Xiaoming;Vernikovskaya,DariaI;Abdelrahman,DoaaR;Hankins,GaryDV;Ahmed,MahmoudS;Nanovskaya,TatianaN
A liquid chromatography in tandem with electro-spray ionization mass spectrometry method has been developed and validated for the quantitative determination of bupropion and its major metabolites (hydroxybupropion, threo- and erythrohydrobupropion) in human umbilical cord plasma and placental tissue. The samples were acidified with trichloroacetic acid, and protein precipitated by adding acetonitrile. Chromatographic separation of drug and metabolites was achieved by using a Waters Symmetry C18column, with an isocratic elution of 31% methanol and 69% formic acid (0.04%, v/v) aqueous solution at a flow rate of 1.0mL/min. Detection was carried out by mass spectrometry using positive electro-spray ionization mode, and the compounds were monitored using multiple reactions monitoring method. Deuterium-labeled isotopes of the compounds were used as internal standards. Calibration curves were linear (r2>0.99) in the tested ranges. The lower limit of quantification of analytes in umbilical cord plasma samples is <0.72ng/mL and 0.92ng/g in placental tissue samples. The relative deviation of this method was <15% for intra- and inter-day assays, and the accuracy ranged between 88% and 105%. The extraction recovery of the four analytes ranged between 89% and 96% in umbilical cord plasma, and 64% and 80% in placental tissue. No significant matrix effect was observed in the presented method.
登录
查看更多内容
影响因子：
1.8
作者：
Parekh, Jignesh M.;Sutariya, Dipen K.;Shrivastav, Pranav S.
通讯作者：
Shrivastav, Pranav S.
DOI：
10.1016/s0731-7085(03)00314-5
发表时间：
2003-09-19
影响因子：
3.4
作者：
Zhang, DD;Yuan, B;Li, FM
通讯作者：
Li, FM
羟基安非他酮的立体选择性分析及其在药物相互作用研究中的应用。
影响因子：
2
作者：
Hongmei Xu;K. Loboz;A. S. Gross;A. McLachlan
通讯作者：
A. McLachlan
影响因子：
5.2
作者：
Schneider, S.;Maul, H.;Poetschke-Langer, M.
通讯作者：
Poetschke-Langer, M.
安非他酮的经胎盘转移和代谢。
DOI：
10.1080/14767050903168424
发表时间：
2010
期刊：
The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians
影响因子：
--
作者：
Earhart,AngelaD;Patrikeeva,Svetlana;Wang,Xiaoming;Abdelrahman,DoaaReda;Hankins,GaryDV;Ahmed,MahmoudS;Nanovskaya,Tatiana
通讯作者：
Nanovskaya,Tatiana