Comparative evaluation of microbial profiles of oral samples obtained at different collection time points and using different methods
Comparative evaluation of microbial profiles of oral samples obtained at different collection time points and using different methods
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DOI：
10.1007/s00784-020-03592-y
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发表时间：
2020-09-25
影响因子：
3.4
通讯作者：
Ueno, Takaaki
中科院分区：
文献类型：
--
作者：
Omori, Michi;Kato-Kogoe, Nahoko;Ueno, Takaaki
目的近年来，口腔微生物群被发现与口腔和全身健康状况有关。虽然有各种口腔样本采集方案可用，但这些方案所产生的结果之间的潜在差异仍不清楚。在这项研究中，我们的目的是确定不同的时间点和口腔样本采集方法对微生物组分析结果的影响。材料与方法采集8名健康成人在进食后2 h、刷牙后即刻、起床后即刻、第二天进食后2 h四个时间点的口腔样本。评估了四种唾液采集方法：吐痰、咀嚼口香糖、棉签和口腔冲洗。通过分析细菌16S rRNA基因序列数据，比较这些样本的口腔微生物群。结果不同采样时间点和采集方法在属水平上的口腔微生物组成基本一致。α多样性在不同组之间没有显著差异，而β多样性在吐痰和棉签方法之间存在差异。与受试者之间的差异相比，组之间的加权UniFrac距离并不小。结论不同采集时间点、不同采集方法所获得的口腔微生物谱相似，但也存在一定的差异。
Objectives Recently, the oral microbiome has been found to be associated with oral and general health status. Although various oral sample collection protocols are available, the potential differences between the results yielded by these protocols remain unclear. In this study, we aimed to determine the effects of different time points and methods of oral sample collection on the outcomes of microbiome analysis. Materials and methods Oral samples were collected from eight healthy individuals at four different time points: 2 h after eating, immediately after teeth brushing, immediately after waking up, and 2 h after eating on the subsequent day. Four methods of saliva collection were evaluated: spitting, gum chewing, cotton swab, and oral rinse. Oral microbiomes of these samples were compared by analyzing the bacterial 16S rRNA gene sequence data. Results The oral microbial composition at the genus level was similar among all sample collection time points and methods. Alpha diversity was not significantly different among the groups, whereas beta diversity was different between the spitting and cotton swab methods. Compared with the between-subject variations, the weighted UniFrac distances between the groups were not minor. Conclusions Although the oral microbiome profiles obtained at different collection time points and using different methods were similar, some differences were detected.