Extremophiles in Deep-Sea Environments
Extremophiles in Deep-Sea Environments
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DOI：
10.1007/978-4-431-67925-7
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发表时间：
2012-10
期刊：
影响因子：
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通讯作者：
K. Horikoshi;K. Tsujii
中科院分区：
文献类型：
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作者：
K. Horikoshi;K. Tsujii
从新西兰北岛陶波湖地区的淡水温泉中分离到一株极端嗜热、硫依赖型古菌WT 1。细胞是有鞭毛的严格厌氧球菌，在85 °C和5.4 g NaCl l-1下生长最佳。 该菌株在复杂的蛋白质底物上或在适当的盐加氨基酸混合物上异养生长，并且还能够利用麦芽糖、淀粉和丙酮酸。元素硫可以被胱氨酸或巯基乙酸盐替代。允许生长的温度范围为60至90 °C;支持生长的pH范围为5至8（最佳pH 7）。 菌株WT 1在含有氨基酸作为唯一碳源和能源的限定培养基中生长。所需氨基酸为：Arg、His、Ile、Leu、Phe、Ser、Thr、Trp、Tyr和瓦尔。菌株WT 1对利福平敏感。DNA G+C含量为50.4mol%。16 S rRNA基因序列的系统发育分析表明，该分离物是一个成员的Thermococcales。DNA/DNA杂交研究表明，没有相似性的几个物种的Thermococcus和Pyrococcus，除了Thermococcus zillipus。根据文献报道的结果，将菌株WT 1命名为怀奥塔普热球菌Thermococcus waiotapuensis sp. nov.
An extremely thermophilic, sulfur-dependent archaeon, strain WT1, was isolated from a freshwater hot spring in the Lake Taupo area of North Island, New Zealand. The cells are flagellated, strictly anaerobic cocci that grow optimally at 85 °C and 5.4 g NaCl l–1. The strain grows heterotrophically on complex proteinaceous substrates or on appropriate salts plus amino acid mixtures and is also able to utilize maltose, starch, and pyruvate. Elemental sulfur could be replaced by cystine or thioglycollate. The range of temperatures allowing growth is from 60 to 90 °C; the pH supporting growth ranges from 5 to 8 (optimum, pH 7). Strain WT1 grew in a defined medium containing amino acids as the sole carbon and energy sources. The required amino acids were: Arg, His, Ile, Leu, Phe, Ser, Thr, Trp, Tyr, and Val. Strain WT1 showed sensitivity to rifampicin. DNA G+C content was 50.4 mol%. Phylogenetic analysis of the sequence encoding the 16S rRNA gene indicated that this isolate is a member of the Thermococcales. DNA/DNA hybridization studies revealed no similarity to several species of Thermococcus and Pyrococcus, with the exception of Thermococcus zilligii. Based on the reported results, we propose strain WT1 as a new species to be named Thermococcus waiotapuensis sp. nov.