Human albumin augmented airway inflammation induced by PM2.5 in NC/Nga mice
Human albumin augmented airway inflammation induced by PM2.5 in NC/Nga mice
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DOI：
10.1002/tox.22751
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发表时间：
2019-07-01
影响因子：
4.5
通讯作者：
Fujikura, Yoshihisa
中科院分区：
文献类型：
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作者：
Nagaoka, Kenjiro;Ogino, Keiki;Fujikura, Yoshihisa
研究了pm2.5和人白蛋白在螨过敏原过敏的NC/Nga小鼠体内的协同变应性炎症作用。腹腔注射PM2.5或PM2.5加人白蛋白加氧化铝2次致敏。7天后，PM2.5或PM2.5加人白蛋白经鼻给药5次，进一步致敏。随后，PM2.5在第11天作为挑战进行管理。第12天，检测小鼠气道高反应性（AHR）、支气管肺泡灌洗液（BALF）细胞计数、肺组织Th-1、Th-2细胞因子、趋化因子、粘液蛋白（MUC5AC、MUC5B） mRNA表达及组织病理学变化。虽然单独使用PM2.5或人白蛋白不会诱发过敏性气道炎症，但同时接种PM2.5和人白蛋白诱导气道炎症表现为AHR、总BALF细胞数、IL-13、eotaxin 1、eotaxin 2和MUC5AC mRNA水平升高，以及对人血清白蛋白的抗ig升高。在PM2.5加人白蛋白诱导的肺中，支气管周围可见炎症。上述结果表明，PM2.5可通过与人白蛋白协同作用诱导NC/Nga小鼠变应性气道炎症。
The synergic allergic inflammatory effects of particulate matter (PM) 2.5 and human albumin were investigated in NC/Nga mice, which are hypersensitive to mite allergens. PM2.5 or PM2.5 plus human albumin with aluminum oxide was injected twice intraperitoneally for sensitization. After 7 days, PM2.5 or PM2.5 plus human albumin was administered five times intranasally to mice for further sensitization. Subsequently, PM2.5 was administered as a challenge on the 11th day. On the 12th day, mice were examined for airway hyperresponsiveness (AHR), bronchoalveolar lavage fluid (BALF) cell count, mRNA expression of Th-1, Th-2 cytokines, chemokines, and mucus proteins (MUC5AC and MUC5B) in the lung tissue and histopathology. Although PM2.5 or human albumin alone did not induce allergic airway inflammation, simultaneous inoculation of PM2.5 and human albumin-induced airway inflammation showing increase in AHR, total BALF cell numbers, mRNA levels of IL-13, eotaxin 1, eotaxin 2, and MUC5AC, and anti-IG against human serum albumin. Inflammation was observed around the bronchus in PM2.5 plus human albumin-induced lungs. These results demonstrate that PM2.5 can induce allergic airway inflammation through the synergistic action with human albumin in NC/Nga mice.