Modular organization analysis of specific naringin/neoeriocitrin related gene expression induced by UVC irradiation in Drynaria roosii
Modular organization analysis of specific naringin/neoeriocitrin related gene expression induced by UVC irradiation in Drynaria roosii
复制标题
UVC照射诱导骨碎补特异性柚皮苷/新圣草次苷相关基因表达的模块化组织分析
DOI：
10.1016/j.envexpbot.2018.09.017
复制
发表时间：
2018-12-01
影响因子：
5.7
通讯作者：
Shi, Lei
中科院分区：
文献类型：
--
作者：
Li, Jing-Yi;Li, Dong;Shi, Lei
槲蕨是一种传统的药用蕨类植物，其根茎中含有有效的黄酮类化合物柚皮苷/新圣草苷。紫外线辐射可诱导植物体内黄酮类化合物的积累，但紫外线辐射介导的柚苷/新圣草苷合成的分子机制尚不清楚。从来没有报道过罗斯病。高效液相色谱-串联质谱（HPLC-MS/MS）分析结果表明，紫外线照射可使D.罗斯。根据NGS数据，基于DEG的热图分析显示，UVC辐射12 h（UV剂量= 81.648 kJ m（-2））比24 h（UV剂量= 163.296 kJ m（-2））更能显著上调柚苷/新圣草苷合成基因，尤其是老根茎中PAL、C3 'H和HCT的表达。通过模块组织分析的系统生物学方法，将15,678个DEG聚类为19个模块，计算模块与样本、模块与NNC之间的相关系数。获得了四个显著的柚苷相关模块，并显示出与特定样品的高度相关性。加权基因共表达网络分析结果表明，4CL、CHS和HCT、C3 H分别作为UVC辐射响应的枢纽基因参与柚苷和新圣草苷的合成，并与MYB/bHLH调控的DEG高水平共表达。总体而言，我们证明了UVC照射上调了单独的新老根茎中的柚苷/新圣草苷相关基因的表达，以剂量依赖的方式增强NNC。激素UVC剂量范围改善D. rosii首次成立。本研究为药用植物次生代谢产物的研究提供了新的思路。
Drynaria roosii is a traditional medicinal fern, whose rhizome is particularly valued for its effective flavonoid components of naringin/neoeriocitrin. UV irradiation can induce flavonoid accumulation in plants, however the molecular mechanism of UVC-mediated naringin/neoeriocitrin synthesis in D. roosii has never been reported. Our HPLC-tandem mass spectrometry (MS/MS) results showed that UVC irradiation increased naringin/neoeriocitrin contents (NNCs) based on irradiation dosage in D. roosii. According to the NGS data, DEG-based heat map analysis revealed up-regulation of naringin/neoeriocitrin synthetic genes was more obvious in 12h UVC irradiation (UV dose = 81.648 kJ m(-2)) when compared with 24h (UV dose = 163.296 kJ m(-2)), especially for PAL, C3'H and HCT in old rhizomes. Through systems biology method of modular organization analysis, we clustered 15,678 DEGs into 19 modules, and calculated correlation coefficients between modules and samples, as well as modules and NNCs. Four significant naringin-related modules were achieved and displayed high correlations with specific samples. Moreover, weighted gene co-expression network analysis results discovered that 4CL, CHS and HCT, C3H acted as the hub responsive genes to UVC irradiation involved in naringin and neoeriocitrin synthesis respectively, and presented high co-expression with MYB/bHLH-regulated DEGs. Overall, we demonstrated that UVC irradiation up-regulated the naringin/neoeriocitrin related gene expression in separate new and old rhizomes to enhance NNCs in a dose-dependent manner. Hormetic UVC dose ranges improving NNCs in D. roosii were established for the first time. Our work also provided new insights into the study of secondary metabolites in medicinal plants.