Development of nuclear microsatellite markers for the Japanese conifers Tsuga diversifolia and T. sieboldii (Pinaceae)
Development of nuclear microsatellite markers for the Japanese conifers Tsuga diversifolia and T. sieboldii (Pinaceae)
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DOI：
10.1111/1442-1984.12182
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发表时间：
2018
影响因子：
1.4
通讯作者：
J. Worth;Jong-Cheol Yang;S. Kanetani;S. Kikuchi;S. Sakaguchi;Tokuko Ihara‐Udino
中科院分区：
文献类型：
--
作者：
J. Worth;Jong-Cheol Yang;S. Kanetani;S. Kikuchi;S. Sakaguchi;Tokuko Ihara‐Udino
为研究日本列岛特有的两种铁杉（Tsuga diversifolia）和南方铁杉（Tsuga diversifolia）的核微卫星标记。sieboldii和与T.韩国郁陵岛上的diversifoliaa。铁杉和T. sieboldii是日本温带和亚高山森林中广泛分布的优势树种，但迄今为止还没有为这些物种开发出遗传标记。15个多态性位点的开发和特点，其中14个是可靠的扩增在每个tax.across两个物种和郁陵岛的人口，每个位点的等位基因数范围从3到26（平均= 13.93）和观察到的杂合度范围从0.005到0.935（平均= 0.535）。此外，所有15个位点都成功地扩增在一个单一的中国物种，T。chinensis。这些标记将有助于研究物种的地理分布、广泛的遗传多样性、保护遗传问题和杂交潜力。
Nuclear microsatellite markers were developed for the twoTsugaspecies native to the Japanese Archipelago,Tsuga diversifoliaandT. sieboldii, and a population with genetic affinities toT. diversifoliaon Ulleung Island, Korea.Tsuga diversifoliaandT. sieboldiiare widespread dominant trees of temperate and subalpine forests in Japan but to date no genetic markers have been developed for these species. Fifteen polymorphic loci were developed and characterized, of which 14 are reliably amplified in each taxon. Across both species and the Ulleung Island population, the number of alleles per locus ranged from 3 to 26 (average = 13.93) and observed heterozygosity ranged from 0.005 to 0.935 (average = 0.535). In addition, all 15 loci were successfully amplified in a single accession of the Chinese species,T. chinensis. These markers will be useful for investigating the species’ biogeography, range‐wide genetic diversity, conservation genetic issues and potential for hybridisation.