Comparative physical mapping between wheat chromosome arm 2BL and rice chromosome 4
Comparative physical mapping between wheat chromosome arm 2BL and rice chromosome 4
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DOI：
10.1007/s10709-010-9528-y
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发表时间：
2010-12-01
期刊：
影响因子：
1.5
通讯作者：
Seo, Yong Weon
中科院分区：
文献类型：
--
作者：
Lee, Tong Geon;Lee, Yong Jin;Seo, Yong Weon
染色体物理图谱为组织和整合不同的遗传信息提供了一个框架。DNA微阵列是一种有价值的物理作图技术，也可用于促进单特征多态(SFP)的发现。利用小麦基因组阵列，利用小麦-水稻共性，将小麦染色体臂2BL物理定位到近等基因系(NILs)上，并定位了小麦EST信息。利用高方差探针集(HVP)分析，共鉴定出314个构成2BL基因的HVP。314个HVP被分成3类：仅与水稻4号染色体匹配的HVP(298个HVP)，仅与2BL(1)上的小麦EST匹配的HVP，以及与2BL(15)上的水稻4号染色体和小麦EST都匹配的HVP(15)。所有的HVP都被转换成基因组，代表独特的水稻基因模型或与识别的HVP匹配的定位小麦EST。构建了小麦16个基因组和水稻271个基因组的比较物理图谱。在271个水稻基因中，有257个基因定位于水稻第4染色体18-35Mb区域。通过HVP分析和水稻染色体基因模型与小麦EST定位的序列相似性，确定了将易位断裂点限制在同源区域的最外层的水稻基因模型。
Physical maps of chromosomes provide a framework for organizing and integrating diverse genetic information. DNA microarrays are a valuable technique for physical mapping and can also be used to facilitate the discovery of single feature polymorphisms (SFPs). Wheat chromosome arm 2BL was physically mapped using a Wheat Genome Array onto near-isogenic lines (NILs) with the aid of wheat-rice synteny and mapped wheat EST information. Using high variance probe set (HVP) analysis, 314 HVPs constituting genes present on 2BL were identified. The 314 HVPs were grouped into 3 categories: HVPs that match only rice chromosome 4 (298 HVPs), those that match only wheat ESTs mapped on 2BL (1), and those that match both rice chromosome 4 and wheat ESTs mapped on 2BL (15). All HVPs were converted into gene sets, which represented either unique rice gene models or mapped wheat ESTs that matched identified HVPs. Comparative physical maps were constructed for 16 wheat gene sets and 271 rice gene sets. Of the 271 rice gene sets, 257 were mapped to the 18-35 Mb regions on rice chromosome 4. Based on HVP analysis and sequence similarity between the gene models in the rice chromosomes and mapped wheat ESTs, the outermost rice gene model that limits the translocation breakpoint to orthologous regions was identified.