Granulocyte-macrophage colony-stimulating factor and tetradecanoyl phorbol acetate induce a distinct, restricted subset of primary-response TIS genes in both proliferating and terminally differentiated myeloid cells.
Granulocyte-macrophage colony-stimulating factor and tetradecanoyl phorbol acetate induce a distinct, restricted subset of primary-response TIS genes in both proliferating and terminally differentiated myeloid cells.
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粒细胞-巨噬细胞集落刺激因子和十四烷酰佛波醇醋酸酯在增殖和终末分化的骨髓细胞中诱导了一个独特的、受限的初级反应 TIS 基因子集。
DOI：
10.1128/mcb.9.8.3580-3583.1989
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发表时间：
1989
影响因子：
5.3
通讯作者：
Herschman,HR
中科院分区：
文献类型：
--
作者：
Varnum,BC;Lim,RW;Kujubu,DA;Luner,SJ;Kaufman,SE;Greenberger,JS;Gasson,JC;Herschman,HR
R. W. Lim， B. C. Varnum, and H. R. Herschman，癌基因1:63- 270,1987)通过粒细胞-巨噬细胞集落刺激因子（GM-CSF）和TPA诱导早期反应基因（tetradecanoyl phorbol acetate [TPA]-诱导序列，或TIS基因）在因子依赖的小鼠细胞系32D克隆3和成熟的人中性粒细胞中进行了研究。当GM-CSF缺失的32D克隆3细胞暴露于GM-CSF或TPA时，4种TIS mrna （TIS7、TIS8、TIS10和TIS11）被快速、短暂地诱导。然而，即使在超诱导条件下，GM-CSF和TPA也不能诱导32D克隆3细胞中TIS1 mRNA的积累。GM-CSF和TPA也能在有丝分裂后的人中性粒细胞中快速、短暂地表达TIS8和TIS11 mRNA。然而，这两种药物都不能诱导TIS1 mRNA在人中性粒细胞中的积累。TIS1是核受体超基因家族的成员，编码配体依赖性转录因子。可诱导转录因子的细胞类型限制可能有助于发育规范。
Induction of early-response genes (tetradecanoyl phorbol acetate [TPA]-induced sequences, or TIS genes; R. W. Lim, B. C. Varnum, and H. R. Herschman, Oncogene 1:263-270, 1987) by granulocyte-macrophage colony-stimulating factor (GM-CSF) and TPA was examined both in a factor-dependent murine cell line, 32D clone 3, and in mature human neutrophils. When GM-CSF-deprived 32D clone 3 cells were exposed to GM-CSF or to TPA, four TIS mRNAs (TIS7, TIS8, TIS10, and TIS11) were rapidly and transiently induced. However, neither GM-CSF nor TPA could induce accumulation of TIS1 mRNA in 32D clone 3 cells, even under superinducing conditions. Both GM-CSF and TPA also elicited rapid, transient expression of TIS8 and TIS11 mRNA in postmitotic human neutrophils. However, neither agent could induce accumulation of TIS1 mRNA in human neutrophils. TIS1 is a member of the nuclear receptor supergene family that codes for ligand-dependent transcription factors. Cell-type restriction of inducible transcription factors may contribute to developmental specification.