Immunogenicity of CTLA4 fusion anti-caries DNA vaccine in rabbits and monkeys
Immunogenicity of CTLA4 fusion anti-caries DNA vaccine in rabbits and monkeys
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CTLA4融合抗龋DNA疫苗兔、猴免疫原性
DOI：
10.1016/j.vaccine.2006.03.090
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发表时间：
2006-06-12
期刊：
影响因子：
5.5
通讯作者：
Xu, Qing An
中科院分区：
文献类型：
--
作者：
Jia, Rong;Guo, Ji Hua;Xu, Qing An
增强粘膜和全身免疫应答仍然是DNA疫苗应用的挑战。在这里，我们展示了抗龋DNA疫苗，pGJA-P和pGJA-P/VAX，编码变形链球菌抗原融合细胞毒性T淋巴细胞抗原-4（CTLA 4），结合抗原呈递细胞表面表达的B7分子。通过鼻内或肌内途径免疫家兔和猴。与非融合DNA疫苗相比，融合DNA疫苗在兔血清和唾液中诱导的特异性抗体应答加速和增加。在融合疫苗免疫的猴中可以检测到显著的特异性血清IgG和唾液伊加水平。因此，这项研究表明，融合抗原CTLA 4的结果，增强免疫效力，并强烈表明，它可能代表一个有前途的方法，以防止龋齿或其他粘膜感染性疾病。CTLA 4融合防龋DNA疫苗可能是灵长类动物有效的免疫原。(c)2006爱思唯尔有限公司保留所有权利。
Enhancement of mucosal and systemic immune responses is still a challenge for the application of DNA vaccine. Here, we show anti-caries DNA vaccines, pGJA-P and pGJA-P/VAX, encoding Streptococcus mutans antigens fused to cytotoxic T lymphocyte antigen-4 (CTLA4), which binds to B7 molecule expressed on the surfaces of antigen-presenting cells. Rabbits and monkeys were immunized via intranasal or intramuscular routes. The fusion vaccine induced accelerated and increased specific antibody responses in serum and saliva compared with non-fusion DNA vaccine in rabbits. Significant specific serum IgG and salivary IgA levels could be detected in fusion vaccine-immunized monkeys. Therefore, this study demonstrates that fusing antigens to CTLA4 results in enhancing immune efficacy and strongly suggests that it may represent a promising approach to prevent dental caries or other mucosal infectious diseases. These findings also suggest that CTLA4 fusion anti-caries DNA vaccine may be effective immunogen in primates. (c) 2006 Elsevier Ltd. All rights reserved.