Physical structures of Tn10-promoted deletions and inversions: Role of 1400 bp inverted repetitions
Physical structures of Tn10-promoted deletions and inversions: Role of 1400 bp inverted repetitions
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Tn10 促进的缺失和倒位的物理结构：1400 bp 倒置重复的作用
DOI：
10.1016/0092-8674(79)90088-6
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发表时间：
1979
期刊：
影响因子：
64.5
通讯作者：
N. Kleckner
中科院分区：
文献类型：
--
作者：
D. G. Ross;J. Swan;N. Kleckner
我们在这里报告了可易位四环素抗性元件TnlO促进的缺失和倒置的物理结构。对噬菌体lambda基因组的DNA/DNA异双工和限制性内切酶分析表明，这两种类型的DNA改变几乎总是起源于TnlO的1400 bp末端反向重复的内部末端。tnlo促进缺失去除从一个这样的末端开始的单个连续DNA片段；TnlO促进的反转更为复杂，涉及TnlO DNA的反转和特异性缺失。
We report here the physical structures of deletions and inversions promoted by the translocatable tetracycline-resistance element TnlO. DNA/DNA heteroduplex and restriction enzyme analyses of alterations in the genome of bacteriophage lambda suggest that both types of DNA alterations almost always originate at the internal termini of the 1400 bp terminal inverted repetitions of TnlO. TnlO-promoted deletions remove a single contiguous DNA segment beginning at one such terminus; TnlO-promated inversions are more complex, and involve both an inversion and a specific deletion of TnlO DNA.