Microfluidic perfusion culture chip providing different strengths of shear stress for analysis of vascular endothelial function
Microfluidic perfusion culture chip providing different strengths of shear stress for analysis of vascular endothelial function
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DOI：
10.1016/j.jbiosc.2014.02.006
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发表时间：
2014-09-01
影响因子：
2.8
通讯作者：
Kanamori, Toshiyuki
中科院分区：
文献类型：
--
作者：
Hattori, Koji;Munehira, Yoichi;Kanamori, Toshiyuki
我们开发了一种微流控灌注细胞培养芯片，它提供了三种不同的剪切应力强度和一个大的细胞培养面积的血管内皮功能的分析。微流控网络由三种不同深度的浅流控通道和深细胞培养通道组成。具有高流体阻力的流动控制通道在细胞培养通道中产生范围为1.0至10.0 dyn/cm（2）的剪切应力强度。培养通道的大表面积能够培养大量（约6.0 × 10（5））细胞。我们培养了人脐静脉内皮细胞（HUVECs），并评估了细胞形态和基因表达的变化，以响应所施加的剪切力。当暴露于10.0 dyn/cm（2）的剪切应力时，HUVEC沿流动方向排列。与无切应力条件相比，在9.5 dyn/cm（2）的切应力下，内皮型一氧化氮合酶mRNA表达增加了50%，血栓调节蛋白mRNA表达增加了8倍。(c)2014年，生物技术学会，日本。All rights reserved.
We developed a microfluidic perfusion cell culture chip that provides three different shear stress strengths and a large cell culture area for the analysis of vascular endothelial functions. The microfluidic network was composed of shallow flow-control channels of three different depths and deep cell culture channels. The flow-control channels with high fluidic resistances created shear stress strengths ranging from 1.0 to 10.0 dyn/cm(2) in the cell culture channels. The large surface area of the culture channels enabled cultivation of a large number (approximately 6.0 x 10(5)) of cells. We cultured human umbilical vein endothelial cells (HUVECs) and evaluated the changes in cellular morphology and gene expression in response to applied shear stress. The HUVECs were aligned in the direction of flow when exposed to a shear stress of 10.0 dyn/cm(2). Compared with conditions of no shear stress, endothelial nitric oxide synthase mRNA expression increased by 50% and thrombomodulin mRNA expression increased by 8-fold under a shear stress of 9.5 dyn/cm(2). (c) 2014, The Society for Biotechnology, Japan. All rights reserved.