Assessing nanoparticle colloidal stability with single-particle inductively coupled plasma mass spectrometry (SP-ICP-MS)
Assessing nanoparticle colloidal stability with single-particle inductively coupled plasma mass spectrometry (SP-ICP-MS)
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DOI：
10.1007/s00216-020-02783-6
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发表时间：
2020-07-06
影响因子：
4.3
通讯作者：
Wilhelm, Stefan
中科院分区：
文献类型：
--
作者：
Donahue, Nathan D.;Francek, Emmy R.;Wilhelm, Stefan
工程纳米粒子的生物相互作用、毒性和环境命运受到胶体稳定性和聚集的影响。为了评估纳米颗粒聚集，需要允许量化单个纳米颗粒聚集体的分析方法。然而，用于纳米颗粒聚集分析的大多数技术限于系综测量或需要苛刻的样品制备，这可能会引入伪影。一个理想的方法将分析聚集体的大小与单纳米粒子的分辨率在原位。在这里，我们建立并验证了单粒子电感耦合等离子体质谱（SP-ICP-MS）作为一种无偏的高通量分析技术，以量化纳米粒子的尺寸分布和聚集在原位。我们通过暴露于生理相关的生理盐水条件诱导纳米颗粒聚集，并应用SP-ICP-MS来量化聚集体大小和在个体聚集体水平的聚集动力学。原位SP-ICP-MS分析揭示了制备胶体稳定纳米颗粒的合理表面工程原理。我们的定量SP-ICP-MS技术是一种平台技术，用于评估各种类型的表面工程纳米颗粒在生理相关条件下的聚集特性。该方法的潜在广泛应用可能包括研究环境样品中的纳米颗粒聚集以及制备用于生物分析测定和纳米医学的胶体稳定的纳米颗粒制剂。
Biological interactions, toxicity, and environmental fate of engineered nanoparticles are affected by colloidal stability and aggregation. To assess nanoparticle aggregation, analytical methods are needed that allow quantification of individual nanoparticle aggregates. However, most techniques used for nanoparticle aggregation analysis are limited to ensemble measurements or require harsh sample preparation that may introduce artifacts. An ideal method would analyze aggregate size in situ with single-nanoparticle resolution. Here, we established and validated single-particle inductively coupled plasma mass spectrometry (SP-ICP-MS) as an unbiased high-throughput analytical technique to quantify nanoparticle size distributions and aggregation in situ. We induced nanoparticle aggregation by exposure to physiologically relevant saline conditions and applied SP-ICP-MS to quantify aggregate size and aggregation kinetics at the individual aggregate level. In situ SP-ICP-MS analysis revealed rational surface engineering principles for the preparation of colloidally stable nanoparticles. Our quantitative SP-ICP-MS technique is a platform technology to evaluate aggregation characteristics of various types of surface-engineered nanoparticles under physiologically relevant conditions. Potential widespread applications of this method may include the study of nanoparticle aggregation in environmental samples and the preparation of colloidally stable nanoparticle formulations for bioanalytical assays and nanomedicine.