Characterization of a Novel HIV-1 CRF01_AE/CRF07_BC Recombinant Strain Among Men Who Have Sex with Men in Liaoning, China
Characterization of a Novel HIV-1 CRF01_AE/CRF07_BC Recombinant Strain Among Men Who Have Sex with Men in Liaoning, China
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中国辽宁男男性行为者中新型 HIV-1 CRF01_AE/CRF07_BC 重组毒株的特征
DOI：
10.1089/aid.2020.0223
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发表时间：
2020-11-05
影响因子：
1.5
通讯作者：
Han, Xiaoxu
中科院分区：
文献类型：
--
作者：
He, Shan;Gao, Yang;Han, Xiaoxu
CRF01_AE和CRF07_BC是中国不同高危人群（包括男男性行为者（MSM））中广泛存在的两种人类免疫缺陷病毒1型（HIV-1）毒株。各种HIV毒株的这种共同流行使得能够产生第二代重组体。本研究从中国辽宁省的一名男男性行为者（MSM）LN 321945中检测到一个新的HIV-1 CRF01_AE/CRF07_BC重组体。系统发育分析和重组分析表明，LN 321945的近全长基因组序列有6个重组断裂点，其中3个CRF07_BC片段插入到CRF01_AE骨架中。进一步的亚区树分析显示，CRF01_AE和CRF07_BC片段均来自MSM人群中两种主要的HIV-1毒株。另外，LN 321945的NFLG基因与另一个在陕西省发现的重组体CRF01_AE/CRF07_BC紧密聚在一起，但这两个重组体具有不同的重组结构和CRF01_AE片段的来源。因此，本研究在MSM人群流行的主要毒株之间鉴定出第二代重组株，表明中国HIV-1流行趋势更加复杂。
CRF01_AE and CRF07_BC are two widespread human immunodeficiency virus type 1 (HIV-1) strains among different high-risk populations, including men who have sex with men (MSM), in China. This co-epidemic of various HIV strains enables the production of second-generation recombinants. In this study, we detected a novel HIV-1 CRF01_AE/CRF07_BC recombinant from LN321945, an MSM lived in Liaoning province, northeast China. The phylogenetic and recombination analyses indicated the near full-length genome (NFLG) sequence of LN321945 had six recombination breakpoints, with three CRF07_BC fragments inserted into a CRF01_AE backbone. Further subregion trees analysis revealed that both CRF01_AE and CRF07_BC fragments were derived from two predominant HIV-1 strains among MSM. In addition, the NFLG of LN321945 was revealed to be clustered closely to another CRF01_AE/CRF07_BC recombinant previously identified in Shaanxi province, northwest China, but these two recombinants had distinct recombination structure and origin of CRF01_AE fragments. Hence, this study identified a second-generation recombinant between the main strains circulating among MSM, indicating more complicated trend of HIV-1 epidemic in China.