Interleukin-10 modulates the sensitivity of peritoneal B lymphocytes to chemokines with opposite effects on stromal cell-derived factor-1 and B-lymphocyte chemoattractant
Interleukin-10 modulates the sensitivity of peritoneal B lymphocytes to chemokines with opposite effects on stromal cell-derived factor-1 and B-lymphocyte chemoattractant
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DOI：
10.1182/blood.v99.2.427
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发表时间：
2002-01-15
期刊：
影响因子：
20.3
通讯作者：
Emilie, D
中科院分区：
文献类型：
--
作者：
Balabanian, K;Foussat, A;Emilie, D
白介素10(IL-10)由腹膜B1a淋巴细胞产生，基质细胞衍生因子-1(SDF-1)由间皮细胞产生。独立研究表明，IL-10和SDF-1都参与了腹膜B淋巴细胞室的持续性。本研究表明，IL-10和SDF-1对腹膜B淋巴细胞有协同作用。事实上，SDF-1对这些细胞的所有作用，包括增加增殖、存活和趋化，都绝对需要IL-10的自分泌产生。此外，在腹膜B淋巴细胞中加入IL-10可增强SDF-1的作用。IL-5、IL-6和IL-9均不影响腹膜B淋巴细胞对SDF-1的反应。IL-10对腹膜B淋巴细胞具有趋化活性，增加其随机迁移率。在不影响CXCR4基因表达的情况下，可增强SDF-1诱导的细胞骨架重组。尽管IL-10具有化学动力学特性，但它能抑制B淋巴细胞趋化因子(BLC)对腹膜B淋巴细胞的迁移，BLC是一种以B淋巴细胞为靶点的趋化因子。B1a淋巴细胞产生IL-10的能力，再加上这种细胞因子对SDF-1和BLC的相反作用，可能解释了B1淋巴细胞在体腔中选择性聚集的原因。(血。2002年；99：427-436)(C)2002，由美国血液病学会主办。
Interleukin-10 (IL-10) is constitutively produced by peritoneal B1a lymphocytes, and stromal cell-derived factor-1 (SDF-1) by mesothelial cells. Independent studies have shown that both IL-10 and SDF-1 are involved in the persistence of the peritoneal B-lymphocyte compartment. This study shows that IL-10 and SDF-1 act in synergy on peritoneal B lymphocytes. Indeed, autocrine production of IL-10 was absolutely required for all effects of SDF-1 on these cells, including increased proliferation, survival, and chemotaxis. Moreover, adding IL-10 to peritoneal B lymphocytes increased the effects of SDF-1. Neither IL-5, IL-6, nor IL-9 affected the response of peritoneal B lymphocytes to SDF-1. IL-10 was chemokinetic for peritoneal B lymphocytes, increasing their random mobility. It also potentiated the SDF-1-induced reorganization of the cytoskeleton without affecting CXCR4 gene expression by peritoneal B lymphocytes. Despite its chemokinetic properties, IL-10 abolished the migration of peritoneal B lymphocytes in response to B-lymphocyte chemoattractant (BLC), a chemokine targeting B lymphocytes to lymphoid organ follicles. The ability of B1a lymphocytes to produce IL-10 constitutively, combined with the opposite effects of this cytokine on the responses to SDF-1 and BLC, may account for the selective accumulation of B1 lymphocytes in body cavities. (Blood. 2002;99:427-436) (C) 2002 by The American Society of Hematology.