Changing P2X receptor localization on maturing sperm in the epididymides of mice, hamsters, rats, and humans: a preliminary study
Changing P2X receptor localization on maturing sperm in the epididymides of mice, hamsters, rats, and humans: a preliminary study
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DOI：
10.1016/j.fertnstert.2009.02.061
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发表时间：
2010-03-15
影响因子：
6.7
通讯作者：
Burnstock, Geoffrey
中科院分区：
文献类型：
--
作者：
Banks, Frederick C. L.;Calvert, Robert C.;Burnstock, Geoffrey
目的：使用免疫组织化学研究人、小鼠、仓鼠和大鼠头部未成熟精子头部P2 X受体亚型的定位设计：基础研究。设置：大学医院。患者：三个人附睾从因转移性前列腺癌接受附睾切除术的患者中获得。主要结果测量：P2 X（1）、P2 X（2）、P2 X（3）和P2 X（4）受体在精子上的免疫定位结果：本研究中，P2 X（1，2）和P2 X（3）受体在人、小鼠、仓鼠和大鼠附睾头的未成熟精子头部均有免疫定位。在小鼠、仓鼠和人类的附睾头中的精子头部也观察到P2 X（4）受体免疫染色，但没有。大鼠除人类的P2 X（4）受体染色持续存在外，随后附睾尾精子上的受体染色消失。与花生凝集素（PNA）结合的研究表明，P2 X受体位于顶体膜上。P2 X（5-7）受体的检测，但发现是absent. Conclusions（s）的变化在本地化的受体亚型是一致的功能上的基本形态和成熟的精子中看到的变化，因为他们通过附睾，并提示的作用，嘌呤能信号在精子的成熟和可能的生育力。（Fertil Steril（R）2010;93：1415-20.（C）2010年由美国生殖医学学会。
Objective: To study using immunohistochemistry the localization of P2X receptor subtypes on the head of immature sperm in the human, mouse, hamster, and rat caputDesign: Basic research.Setting: University-based hospital.Patient(s): Three human epididymides were obtained from patients undergoing orchidectomy for metastatic prostate cancer.Main Outcome Measure(s): P2X(1), P2X(2), P2X(3), and P2X(4) receptor immunolocalization on sperm.Result(s): In the present study, P2X(1,2), (and 3) receptor localization was immunohistochemically demonstrated on the head of immature sperm in the human, mouse, hamster, and rat caput epididymidis. P2X(4) receptor immunostaining was also observed on the head of sperm in the caput epididymidis of mice, hamsters, and humans, but not. rats. There was a subsequent loss of receptor staining on sperm in the cauda epididymidis, except in humans where staining of P2X(4) receptors persisted. Comparision with peanut agglutinin (PNA) binding studies suggested the P2X receptors were located on the acrosome membrane. P2X(5-7) receptors were examined but found to be absent.Conclusion(s): The change in localization of receptor subtypes is coincidental with the functionally essential morphologic and maturational changes seen in sperm as they travel through the epididymis, and is suggestive of a role for purinergic signaling in sperm maturation and possibly fertility. (Fertil Steril (R) 2010;93:1415-20. (C)2010 by American Society for Reproductive Medicine.)