Different Protocols to Produce Artificial Dentine Carious Lesions in vitro and in situ: Hardness and Mineral Content Correlation
Different Protocols to Produce Artificial Dentine Carious Lesions in vitro and in situ: Hardness and Mineral Content Correlation
复制标题
DOI：
10.1159/000345362
复制
发表时间：
2013-01-01
期刊：
影响因子：
4.2
通讯作者：
Magalhaes, A. C.
中科院分区：
文献类型：
--
作者：
Moron, B. M.;Comar, L. P.;Magalhaes, A. C.
本研究比较了体外模型和原位模型诱导的牙本质脱矿，并通过横向显微摄影(TMR)比较了牙本质表面硬度(SH)、截面硬度(CSH)和矿物质含量之间的关系。用MC凝胶(6%羧甲基纤维素凝胶和0.1M乳酸，pH 5.0，14d)、缓冲液I(0.05M醋酸钙、磷酸盐和氟化钠溶液，pH 4.5，7d)、缓冲液11(0.05M醋酸钙和磷酸盐溶液，pH 5.0，7d)和TEMDP(0.05M乳酸、钙、磷酸盐和四甲基二膦酸乙酯，pH 5.0，7d)对牛牙本质标本进行体外脱矿。在一项现场研究中，11名志愿者佩戴了包含2个牛牙本质标本的腭部矫治器，用塑料网保护，使生物膜得以形成。志愿者将20%的蔗糖溶液滴在每个标本上，每天4次，连续14天。使用TMR对体外和原位病变进行分析，并用方差分析进行统计学比较。对TMR和CSH/SH进行回归分析和相关分析(p<；0.05)。原位模型产生了深部病变，R值较高，但表层较薄。在体外模型方面，MC凝胶仅产生浅层损伤，而缓冲液I和II以及TEMDP诱导了明显的表层下损伤和深度脱矿。CSH与TMR之间的关系较弱，且不是线性关系。不同模型诱导的人工牙本质龋损明显不同，进而可能影响进一步的去矿化和再矿化过程。硬度分析不应解释为牙本质矿物质丢失。版权所有(C)2012 S.Karger AG，巴塞尔
This study compared dentine demineralization induced by in vitro and in situ models, and correlated dentine surface hardness (SH), cross-sectional hardness (CSH) and mineral content by transverse microradiography (TMR). Bovine dentine specimens (n = 15/group) were demineralized in vitro with the following: MC gel (6% carboxymethylcellulose gel and 0.1 M lactic acid, pH 5.0, 14 days); buffer I (0.05 M acetic acid solution with calcium, phosphate and fluoride, pH 4.5, 7 days); buffer 11 (0.05 M acetic acid solution with calcium and phosphate, pH 5.0, 7 days), and TEMDP (0.05 M lactic acid with calcium, phosphate and tetraethyl methyl diphosphonate, pH 5.0, 7 days). In an in situ study, 11 volunteers wore palatal appliances containing 2 bovine dentine specimens, protected with a plastic mesh to allow biofilm development. The volunteers dripped a 20% sucrose solution on each specimen 4 times a day for 14 days. In vitro and in situ lesions were analyzed using TMR and statistically compared by ANOVA. TMR and CSH/SH were submitted to regression and correlation analysis (p < 0.05). The in situ model produced a deep lesion with a high R value, but with a thin surface layer. Regarding the in vitro models, MC gel produced only a shallow lesion, while buffers I and II as well as TEMDP induced a pronounced subsurface lesion with deep demineralization. The relationship between CSH and TMR was weak and not linear. The artificial dentine carious lesions induced by the different models differed significantly, which in turn might influence further de- and remineralization processes. Hardness analysis should not be interpreted with respect to dentine mineral loss. Copyright (C) 2012 S. Karger AG, Basel