Spatial regulation of RhoC activity defines protrusion formation in migrating cells
Spatial regulation of RhoC activity defines protrusion formation in migrating cells
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DOI：
10.1242/jcs.123547
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发表时间：
2013-08-01
影响因子：
4
通讯作者：
Hodgson, Louis
中科院分区：
文献类型：
--
作者：
Bravo-Cordero, Jose Javier;Sharma, Ved P.;Hodgson, Louis
突起的形成是运动细胞运动前的第一步。在细胞迁移过程中，肌动蛋白聚合的空间控制是实现定向突起的必要条件。研究表明，空间协调子p190RhoGEF和p190RhoGAP通过调节肌动蛋白倒钩端分布和振幅来调节前缘突起过程中的肌动蛋白聚合。p190RhoGEF和p190RhoGAP实现的RhoC活性分布和cofilin激活的适当平衡决定了最终突出活性的方向。这些发现为研究倒刺末端的振幅和分布的动态可塑性提供了新的见解，倒刺末端的动态可塑性可以通过上游gef和gap微调RhoC活性来调节，从而实现定向细胞运动。
Protrusion formation is the first step that precedes cell movement of motile cells. Spatial control of actin polymerization is necessary to achieve directional protrusion during cell migration. Here we show that the spatial coordinators p190RhoGEF and p190RhoGAP regulate actin polymerization during leading edge protrusions by regulating the actin barbed end distribution and amplitude. The distribution of RhoC activity and proper balance of cofilin activation achieved by p190RhoGEF and p190RhoGAP determines the direction of final protrusive activity. These findings provide a new insight into the dynamic plasticity in the amplitude and distribution of barbed ends, which can be modulated by fine-tuning RhoC activity by upstream GEFs and GAPs for directed cell motility.