Design of multi-functional linear polymers that capture and neutralize a toxic peptide: a comparison with cross-linked nanoparticles
Design of multi-functional linear polymers that capture and neutralize a toxic peptide: a comparison with cross-linked nanoparticles
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捕获和中和有毒肽的多功能线性聚合物的设计：与交联纳米粒子的比较
DOI：
10.1039/c4tb01967a
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发表时间：
2015
影响因子：
7
通讯作者：
Yoshiko Miura
中科院分区：
文献类型：
--
作者：
Yusuke Wada;Haejoo Lee, Yu Hoshino, Shunsuke Kotani;Kenneth J. Shea;Yoshiko Miura
在本文中，多功能的线性聚-N-异丙基丙烯酰胺（pNIPAm）聚合物具有一系列的分子量和官能团的图书馆，合成和它们的相互作用与溶血肽，蜂毒肽，进行了检查。含有叔丁基和羧酸的线性pNIPAm（LPs）通过疏水和静电相互作用与肽结合并中和其毒性。定量每种LP的蜂毒肽结合能力和亲和力，并进一步与具有相同官能团组合的交联多功能纳米凝胶颗粒（NP）进行比较。的LP的结合能力（捕获的蜂毒肽的重量/LP的重量）是独立的，其分子量和是三倍高于先前报道的NP。结合常数取决于脂多糖的分子量，对于含有40%叔丁基和20%羧酸的约1000 mer线性聚合物，结合常数最高为1.1 × 108（M−1）。LP和NP的相互作用的比较表明聚合物链的柔性对于实现高结合能力和亲和力的重要性。
In this paper, a library of multi-functional linear poly-N-isopropylacrylamide (pNIPAm) polymers having a range of molecular weights and functional groups were synthesized and their interaction with the hemolytic peptide, melittin, was examined. The linear pNIPAm (LPs) containing both tert-butyl group and carboxylic acids bound with the peptide by a combination of hydrophobic and electrostatic interactions and neutralized its toxicity. The melittin binding capacity and affinity of each LP was quantified and further compared with cross-linked multi-functional nanogel particles (NPs) having same combination of functional groups. The binding capacity of the LPs (weight of captured melittin/weight of LP) was independent of their molecular weight and was three times higher than that of previously reported NPs. The binding constant depended on the molecular weight of the LPs, showing the highest value of 1.1 × 108 (M−1) for a ∼1000 mer linear polymer with 40% tert-butyl group and 20% carboxylic acid. Comparison of the interactions of the LPs and NPs suggested the importance of the flexibility of the polymer chain in order to achieve high binding capacity and affinity.