Evaluation of Sysmex XN-1000 High-Sensitive Analysis (hsA) Research Mode for Counting and Differentiating Cells in Cerebrospinal Fluid
Evaluation of Sysmex XN-1000 High-Sensitive Analysis (hsA) Research Mode for Counting and Differentiating Cells in Cerebrospinal Fluid
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DOI：
10.1093/ajcp/aqv093
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发表时间：
2016-03-01
影响因子：
3.5
通讯作者：
de Jonge, Robert
中科院分区：
文献类型：
--
作者：
Fleming, Cherina;Russcher, Henk;de Jonge, Robert
目的：使用自动分析仪对脑脊液 (CSF) 中的细胞进行计数通常存在问题，因为细胞数量较少时精度较低。为了克服这一限制，Sysmex（日本神户）开发了高灵敏度分析 (hsA) 研究模式，专门用于对细胞计数较低的液体中的细胞进行计数。我们通过计数 CSF 样本中的 RBC、WBC 和分化 WBC 来评估该模式。方法：我们使用 hsA 模式分析了 248 个 CSF 样本，并将这些结果与使用手动计数方法获得的结果进行比较。我们还评估了 hsA 模式的线性、检测限、残留和精密度。结果：使用 hsA 模式，RBC 和 WBC 的定量下限分别为 10 和 2 个细胞/μL。比较两种方法显示，WBC (y = 1.08x + 0.52)、RBC (y = 1.07x + 0.00)、淋巴细胞 (y = 1.00x + 0.00)、中性粒细胞 (y = 1.05x + 0.00) 和单核细胞 (y = 0.88x + 0.07) 具有良好的一致性。对含有低 WBC 的样品进行回归分析（
Objectives: Counting cells in cerebrospinal fluid (CSF) using automated analyzers is generally problematic due to low precision at low cell numbers. To overcome this limitation, Sysmex (Kobe, Japan) developed the high-sensitive analysis (hsA) research mode specifically for counting cells in fluids that contain low cell counts. We evaluated this mode by counting RBCs, WBCs, and differentiated WBCs in CSF samples.Methods: We analyzed 248 CSF samples using the hsA mode and compared these results with those obtained using the manual counting method. We also evaluated the linearity, detection limits, carryover, and precision of the hsA mode.Results: Using the hsA mode, the lower limit of quantification for RBCs and WBCs was 10 and 2 cells/mu L, respectively. Comparing the two methods revealed good agreement with respect to WBCs (y = 1.08x + 0.52), RBCs (y = 1.07x + 0.00), lymphocytes (y = 1.00x + 0.00), neutrophils (y = 1.05x + 0.00), and monocytes (y = 0.88x + 0.07). Regression analysis for samples containing low WBCs (