PRIMARY AND SECONDARY STRUCTURES OF ESCHERICHIA-COLI MRE-600-23S RIBOSOMAL-RNA - COMPARISON WITH MODELS OF SECONDARY STRUCTURE FOR MAIZE CHLOROPLAST 23S RIBOSOMAL-RNA AND FOR LARGE PORTIONS OF MOUSE AND HUMAN 16S MITOCHONDRIAL RIBOSOMAL-RNAS
PRIMARY AND SECONDARY STRUCTURES OF ESCHERICHIA-COLI MRE-600-23S RIBOSOMAL-RNA - COMPARISON WITH MODELS OF SECONDARY STRUCTURE FOR MAIZE CHLOROPLAST 23S RIBOSOMAL-RNA AND FOR LARGE PORTIONS OF MOUSE AND HUMAN 16S MITOCHONDRIAL RIBOSOMAL-RNAS
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DOI：
10.1093/nar/9.17.4303
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发表时间：
1981-01-01
影响因子：
14.9
通讯作者：
KOSSEL, H
中科院分区：
文献类型：
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作者：
BRANLANT, C;KROL, A;KOSSEL, H
我们用测序凝胶技术对E.coliMRE 600 23S rRNA的Tl、Sl、A和Naja氧核酸酶的酶切产物进行了测序，确定了其90%的一级结构。检测到8个顺反子异质性，并与已发表的E.coliK12株23S rRNA基因序列有16个差异。确定了E.coli23S rRNA转录后修饰的13个核苷酸以及单链、双链和亚单位表面区的位置。利用这些实验结果，通过比较E.coli23S rRNA、玉米Clo的序列。23S rRNA以及鼠和人的MIT 16S rRNA，我们为两个23S rRNA和两个MIT rRNA的很大一部分建立了二级结构模型。玉米叶绿体和E.Coli23S rRNAs的结构非常相似，由7个结构域组成，由远程碱基配对封闭。在线粒体的16S rRNAs中，有3个结构域的大小明显缩小，与23S rRNA的一级结构完全不同。这些结构域以前被发现在E.coli50S亚基中构成一个紧凑的区域。保守结构域不属于该区域，包含几乎所有的修饰核苷酸。最保守的结构域，2042-2625，可能是核糖体A位点的一部分。最后，我们的研究强烈表明，在细胞质核糖体中，5.8S rRNA的3‘端与26S或28S rRNA的5’端是碱基配对的。这证实了5.8S RNA是原核生物23S rRNA 5‘末端区域的对应物。
We determined 90% of the primary structure ofE.coliMRE 600 23S rRNA by applying the sequencing gel technique to products of Tl, Sl, A andNaja oxiananuclease digestion. Eight cistron heterogeneities were detected, as well as 16 differences with the published sequence of a 23S rRNA gene of anE.coliK12 strain. The positions of 13 post-transcriptionally modified nucleotides and of single-stranded, double-stranded and subunit surface regions ofE.coli23S rRNA were identified. Using these experimental results and by comparing the sequences ofE.coli23S rRNA, maize chloro. 23S rRNA and mouse and human mit 16S rRNAs, we built models of secondary structure for the two 23S rRNAs and for large portions of the two mit rRNAs . The structures proposed for maize chloroplast and E.coli 23S rRNAs are very similar, consisting of 7 domains closed by long-range base-pairings. In the mitochondrial 16S rRNAs, 3 of these domains are strongly reduced in size and have a very different primary structure compared to those of the 23S rRNAs. These domains were previously found to constitute a compact area in theE.coli50S subunits. The conserved domains do not belong to this area and contain almost all the modified nucleotides. The most highly conserved domain, 2042–2625, is probably part of the ribosomal A site. Finally, our study strongly suggests that in cytoplasmic ribosomes the 3′-end of 5.8S rRNA is base-paired with the 5′-end of 26S or 28S rRNA. This confirms the idea that 5.8S RNA is the counterpart of the 5′-terminal region of prokaryotic 23S rRNA.