Targeting Inhibitory Chondroitin Sulphate Proteoglycans to Promote Plasticity After Injury
Targeting Inhibitory Chondroitin Sulphate Proteoglycans to Promote Plasticity After Injury
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DOI：
10.1007/978-1-4939-0777-9_10
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发表时间：
2014-01-01
期刊：
影响因子：
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通讯作者：
Fawcett, James W.
中科院分区：
文献类型：
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作者：
Kwok, Jessica C. F.;Heller, Janosch P.;Fawcett, James W.
硫酸软骨素蛋白多糖（CSPGs）是抑制中枢神经系统细胞外基质分子的主要家族之一。各种CSPGs在神经系统发育早期表达强烈；然而，它在成熟过程中下调，在神经系统损伤后再次上调。在体内注射一种名为软骨素酶ABC的酶，它可以去除CSPGs上的抑制性硫酸软骨素链，在损伤区域促进神经元的再生和可塑性。在此，我们描述了将软骨素酶ABC体内注射到成年大鼠脑皮层的方法和组织化学方法来评估消化成功。
Chondroitin sulphate proteoglycans (CSPGs) are one of the major families of inhibitory extracellular matrix molecules in the central nervous system. The expression of various CSPGs is strong during early nervous system development; however, it is downregulated during maturation and up-regulated again after nervous system injury. In vivo injection of an enzyme called chondroitinase ABC, which removes the inhibitory chondroitin sulphate chains on the CSPGs, in the injured area promotes both the regeneration and plasticity of the neurons. Here, we describe the method of in vivo injection of the chondroitinase ABC into the cortex of adult rat brain and the histochemical method to assess the successfulness of the digestion.