Salphen metal complexes as tunable G-quadruplex binders and optical probes
Salphen metal complexes as tunable G-quadruplex binders and optical probes
复制标题
DOI：
10.1039/c3ra44793f
复制
发表时间：
2014-01-01
期刊：
影响因子：
3.9
通讯作者：
Vilar, Ramon
中科院分区：
文献类型：
--
作者：
Karim, Nurul H. Abd;Mendoza, Oscar;Vilar, Ramon
合成了一系列新的金属配合物（金属= Ni-II，Cu-II，PtII和VO ~（2+）），并测定了它们对DNA的双链体和G-四链体的亲和力。复合物对给定DNA拓扑结构的选择性由几个因素决定，包括复合物的几何形状、总电荷和取代模式。我们还表明，作为本系列的一部分开发的两种铂（II）-水杨酸配合物具有发射性。使用四种不同的细胞系（CHO、HeLa、U2 OS和HepG 2）用这两种复合物进行共聚焦显微镜研究。这些研究表明，两种探针的细胞渗透性和定位是不同的，并且高度依赖于所使用的细胞系。
A series of seven new metal complexes (metal = Ni-II, Cu-II, PtII and VO2+) with substituted salphen ligands have been prepared and their duplex and G-quadruplex DNA affinities determined. The selectivity of the complexes towards a given DNA topology is dictated by several factors including geometry, overall charge and substitution pattern of the complex. We also show that the two platinum(II)-salphen complexes developed as part of this series are emissive. Confocal microscopy studies were carried out with these two complexes using four different cell lines (CHO, HeLa, U2OS and HepG2). These studies showed that the cell permeability and localization are different for the two probes and highly dependent on the cell line used.