Production of isoprene, one of the high-density fuel precursors, from peanut hull using the high-efficient lignin-removal pretreatment method.
Production of isoprene, one of the high-density fuel precursors, from peanut hull using the high-efficient lignin-removal pretreatment method.
复制标题
采用高效脱木质素预处理方法从花生壳中生产异戊二烯，高密度燃料前体之一
DOI：
10.1186/s13068-017-0988-5
复制
发表时间：
2017
影响因子：
6.3
通讯作者：
Xian M
中科院分区：
文献类型：
--
作者：
Wang S;Wang Z;Wang Y;Nie Q;Yi X;Ge W;Yang J;Xian M
异戊二烯作为原料可以用来生产可再生能源燃料，为迅速枯竭的化石燃料提供了替代品。然而，传统的异戊二烯生产方法已不能满足低能耗、环境友好的要求。此外，以前的研究大多集中在木质纤维素材料，如木材，稻草，玉米芯生产生物燃料，而很少有研究集中在使用花生船体（PH）生产生物燃料。众所周知，中国是地球仪上最大的花生生产国，每年的花生产量极其可观。因此，一种新的，可再生的，和环境友好的预处理策略，以提高纤维素的酶解效率和减少抑制剂的产生，开发转化为isopropyl.ResultsThe最佳预处理条件为100 °C，60 min，10%（w/v）的固体负载与2：8体积比的磷酸和过氧化氢。半纤维素和木质素含量分别降低到85.0%和98.0%。纤维素-葡萄糖转化率由19.1%提高到95.0%。在预处理过程中只生成了甲酸、乙酰丙酸和少量糠醛3种抑制剂，其浓度较低，不足以抑制大肠杆菌发酵异戊二烯的产量。与纯葡萄糖发酵的异戊二烯产量（298 ± 9 mg/L）相比，工程菌以预处理后的PH为碳源，分别水解发酵和同步糖化发酵（SSF）的异戊二烯产量分别为249 ± 6.7和294 ± 8.3 mg/L。通过SSF生产异戊二烯有9.8%的葡萄糖-异戊二烯转化率，相当于98.8%的异戊二烯生产通过纯葡萄糖发酵conclusionsThe优化的磷酸/过氧化氢组合预处理方法被证明是有效的去除木质纤维素和半纤维素材料。同时，预处理后的PH值在工程菌中能高效转化为异戊二烯.研究表明，磷酸/过氧化氢预处理木质纤维素原料生产异戊二烯是一种很有前途的替代传统方法，可以充分利用不可再生的化石资源。
BackgroundIsoprene as the feedstock can be used to produce renewable energy fuels, providing an alternative to replace the rapidly depleting fossil fuels. However, traditional method for isoprene production could not meet the demands for low-energy consumption and environment-friendliness. Moreover, most of the previous studies focused on biofuel production out of lignocellulosic materials such as wood, rice straw, corn cob, while few studies concentrated on biofuel production using peanut hull (PH). As is known, China is the largest peanut producer in the globe with an extremely considerable amount of PH to be produced each year. Therefore, a novel, renewable, and environment-friendly pretreatment strategy to increase the enzymatic hydrolysis efficiency of cellulose and reduce the inhibitors generation was developed to convert PH into isoprene.ResultsThe optimal pretreatment conditions were 100 °C, 60 min, 10% (w/v) solid loading with a 2:8 volume ratio of phosphoric acid and of hydrogen peroxide. In comparison with the raw PH, the hemicellulose and lignin were reduced to 85.0 and 98.0%, respectively. The cellulose–glucose conversion of pretreated PH reached up to 95.0% in contrast to that of the raw PH (19.1%). Only three kinds of inhibitors including formic acid, levulinic acid, and a little furfural were formed during the pretreatment process, whose concentrations were too low to inhibit the isoprene yield forEscherichia colifermentation. Moreover, compared with the isoprene yield of pure glucose fermentation (298 ± 9 mg/L), 249 ± 6.7 and 294 ± 8.3 mg/L of isoprene were produced using the pretreated PH as the carbon source by the engineered strain via separate hydrolysis and fermentation and simultaneous saccharification and fermentation (SSF) methods, respectively. The isoprene production via SSF had a 9.8% glucose–isoprene conversion which was equivalent to 98.8% of isoprene production via the pure glucose fermentation.ConclusionsThe optimized phosphoric acid/hydrogen peroxide combination pretreatment approach was proved effective to remove lignin and hemicellulose from lignocellulosic materials. Meanwhile, the pretreated PH could be converted into isoprene efficiently in the engineeredEscherichia coli. It is concluded that this novel strategy of isoprene production using lignocellulosic materials pretreated by phosphoric acid/hydrogen peroxide is a promising alternative to isoprene production using traditional way which can fully utilize non-renewable fossil sources.
登录
查看更多内容
DOI：
10.1016/j.jiec.2013.10.056
发表时间：
2014-09-25
影响因子：
6.1
作者：
Hong, In Kwon;Jeon, Hyeon;Lee, Seung Bum
通讯作者：
Lee, Seung Bum
影响因子：
3
作者：
Alizadeh, H;Teymouri, F;Dale, BE
通讯作者：
Dale, BE
影响因子：
11.4
作者：
da Costa Correia, Jessyca Aline;Marques Junior, Jose Edvan;Ponte Rocha, Maria Valderez
通讯作者：
Ponte Rocha, Maria Valderez
影响因子：
11.4
作者：
Kim, Ho Myeong;Wi, Seung Gon;Bae, Hyeun-Jong
通讯作者：
Bae, Hyeun-Jong
影响因子：
9.8
作者：
Matos, Cristina T.;Gouveia, Luisa;Bogel-Lukasik, Rafal
通讯作者：
Bogel-Lukasik, Rafal