Lights, camera, action! Capturing the spliceosome and pre-mRNA splicing with single-molecule fluorescence microscopy.
Lights, camera, action! Capturing the spliceosome and pre-mRNA splicing with single-molecule fluorescence microscopy.
复制标题
灯，相机，动作！用单分子荧光显微镜捕获剪接体和前MRNA剪接。
DOI：
10.1002/wrna.1358
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发表时间：
2016-09
期刊：
影响因子：
--
通讯作者：
Hoskins AA
中科院分区：
文献类型：
--
作者：
DeHaven AC;Norden IS;Hoskins AA
The process of removing intronic sequences from a precursor to messenger RNA (pre-mRNA) to yield a mature mRNA transcript via splicing is an integral step in eukaryotic gene expression. Splicing is carried out by a cellular nanomachine called the spliceosome that is composed of RNA components and dozens of proteins. Despite decades of study, many fundamentals of spliceosome function have remained elusive. Recent developments in single molecule fluorescence microscopy have afforded new tools to better probe the spliceosome and the complex, dynamic process of splicing by direct observation of single molecules. These cutting-edge technologies enable investigators to monitor the dynamics of specific splicing components, whole spliceosomes, and even co-transcriptional splicing within living cells.
