Detection of Phosphatidylserine Exposure on Leukocytes Following Treatment with Human Galectins.
Detection of Phosphatidylserine Exposure on Leukocytes Following Treatment with Human Galectins.
复制标题
用人半乳糖凝集素处理后白细胞上磷脂酰丝氨酸暴露的检测。
DOI：
10.1007/978-1-0716-2055-7_28
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Arthur,ConnieM
中科院分区：
文献类型：
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作者：
Stowell,SeanR;Dias-Baruffi,Marcelo;Cummings,RichardD;Arthur,ConnieM
细胞更新是免疫稳态的一个基本方面。虽然许多因素似乎在炎症消退期间调节白细胞去除，但最近的研究表明，半乳糖凝集素家族的成员在协调这一过程中发挥着独特的作用。与通过凋亡性细胞死亡的细胞去除不同，在没有细胞死亡的情况下，半乳糖凝集素家族的几个成员诱导磷脂酰丝氨酸（PS）的表面表达，PS是经历凋亡的细胞上的吞噬标志物。然而，类似于PS对细胞凋亡，半乳糖凝集素诱导的PS曝光敏感的细胞吞噬清除。由于半乳糖凝集素似乎准备吞噬细胞的去除，而实际上不诱导凋亡细胞死亡，这个过程最近被创造的preaparesis。鉴于半乳糖凝集素诱导的PS暴露在血液分离前的背景下的独特特征，我们将在评估不同半乳糖凝集素家族成员对PS暴露和细胞活力的潜在影响时检查独特的考虑因素。
Cellular turnover represents a fundamental aspect of immunological homeostasis. While many factors appear to regulate leukocyte removal during inflammatory resolution, recent studies suggest that members of the galectin family play a unique role in orchestrating this process. Unlike cellular removal through apoptotic cell death, several members of the galectin family induce surface expression of phosphatidylserine (PS), a phagocytic marker on cells undergoing apoptosis, in the absence of cell death. However, similar to PS on cells undergoing apoptosis, galectin-induced PS exposure sensitizes cells to phagocytic removal. As galectins appear to prepare cells for phagocytic removal without actually inducing apoptotic cell death, this process has recently been coined preaparesis. Given the unique characteristics of galectin-induced PS exposure in the context of preaparesis, we will examine unique considerations when evaluating the potential impact of different galectin family members on PS exposure and cell viability.