Effects of monochromatic light on mucosal mechanical and immunological barriers in the small intestine of broilers
Effects of monochromatic light on mucosal mechanical and immunological barriers in the small intestine of broilers
复制标题
单色光对肉鸡小肠粘膜机械和免疫屏障的影响
DOI：
10.3382/ps.2011-01416
复制
发表时间：
2011-12-01
期刊：
影响因子：
4.4
通讯作者：
Chen, Y. X.
中科院分区：
文献类型：
--
作者：
Xie, D.;Li, J.;Chen, Y. X.
我们之前的研究表明，绿色和蓝色单色光可以有效刺激肉鸡脾脏的免疫反应。本研究旨在研究单色光对肠粘膜机械和免疫屏障的影响。孵化后第 0 天（P）的总共 120 只 Arbor Acre 雄性肉鸡分别暴露在红光、绿光（GL）、蓝光（BL）和白光（WL）下 49 d。与暴露于WL的肉鸡相比，暴露于GL的肉鸡小肠绒毛高度增加了19.5％（P = 0.0205）和38.8％（P = 0.0149），小肠隐窝深度减少了15.1％（P = 0.0049）和10.1％（P = 0.0005），并且比率P7 和 P21 时，绒毛高度与隐窝深度的比值分别增加了 39.3% (P < 0.0001) 和 52.5% (P < 0.0001)。直到 P49，与 WL 组相比，BL 组的绒毛高度增加（33.6%，P = 0.0076），隐窝深度减少（15.4%，P = 0.0201），绒毛高度与隐窝深度之比增加（58.5%，P < 0.0001）。另一方面，肠上皮内淋巴细胞（27.9%，P < 0.0001 和 37.0%，P < 0.0001）、杯状细胞（GC，22.1%，P < 0.0001 和 18.1%，P < 0.0001）和 IgA(+) 细胞（14.8%，P = 0.0543 和与 WL 组相比，GL 组小肠中的 47.9%，P = 0.0377 分别在 P7 和 P21 时显着增加。 P49时BL组肠上皮内淋巴细胞（36.2%，P < 0.0001）、GC（26.5%，P < 0.0001）和IgA（+）细胞（68.0%，P = 0.0177）数量也高于WL组。这些结果表明，通过在幼年时在 GL 下饲养肉鸡并在较大年龄时在 BL 下饲养，可以改善小肠的粘膜机械和免疫屏障。
Our previous studies demonstrated that green and blue monochromatic lights were effective to stimulate immune response of the spleen in broilers. This study was designed to investigate the effects of monochromatic light on both gut mucosal mechanical and immunological barriers. A total of 120 Arbor Acre male broilers on post-hatching day (P) 0 were exposed to red light, green light (GL), blue light (BL), and white light (WL) for 49 d, respectively. As compared with broilers exposed to WL, the broilers exposed to GL showed that the villus height of small intestine was increased by 19.5% (P = 0.0205) and 38.8% (P = 0.0149), the crypt depth of small intestine was decreased by 15.1% (P = 0.0049) and 10.1% (P = 0.0005), and the ratios of villus height to crypt depth were increased by 39.3% (P < 0.0001) and 52.5% (P < 0.0001) at P7 and P21, respectively. Until P49, an increased villus height (33.6%, P = 0.0076), a decreased crypt depth (15.4%, P = 0.0201), and an increased villus height-to-crypt depth ratio (58.5%, P < 0.0001) were observed in the BL group as compared with the WL group. On the other hand, the numbers of intestinal intraepithelial lymphocytes (27.9%, P < 0.0001 and 37.0%, P < 0.0001), goblet cells (GC, 22.1%, P < 0.0001 and 18.1%, P < 0.0001), and IgA(+) cells (14.8%, P = 0.0543 and 47.9%, P = 0.0377) in the small intestine were significantly increased in the GL group as compared with the WL group at P7 and P21, respectively. The numbers of intestinal intraepithelial lymphocytes (36.2%, P < 0.0001), GC (26.5%, P < 0.0001), and IgA(+) cells (68.0%, P = 0.0177) in the BL group were also higher than those in the WL group at P49. These results suggest that both mucosal mechanical and immunological barriers of the small intestine may be improved by rearing broilers under GL at an early age and under BL at an older age.