Effects of soluble laminin on organelle transport and neurite growth in cultured mouse dorsal root ganglion neurons: Difference between primary neurites and branches
Effects of soluble laminin on organelle transport and neurite growth in cultured mouse dorsal root ganglion neurons: Difference between primary neurites and branches
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DOI：
10.1002/jcp.20394
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发表时间：
2005-11
影响因子：
5.6
通讯作者：
K. Kohno;T. Kawakami;H. Hiruma
中科院分区：
文献类型：
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作者：
K. Kohno;T. Kawakami;H. Hiruma
层粘连蛋白是一种细胞外基质分子，已知可促进神经突生长。在本研究中，可溶性层粘连蛋白的细胞器运输的影响及其与突起生长的关系在培养的分离的小鼠背根神经节（DRG）神经元进行了研究。层粘连蛋白加入到细胞外培养液中，可沉积在DRG神经元表面。与可溶性层粘连蛋白孵育的DRG神经元表现出分支，长，薄的神经突起。时间推移研究表明，加入层粘连蛋白后，新形成了许多小直径分支。因此，单独分析了由原发性神经炎生长锥延伸的细胞体和分支引起的大直径原发性神经炎的生长。层粘连蛋白抑制初生突起的生长，但促进分支的生长。在初级神经突中，层粘连蛋白的急性加入迅速降低了神经突轴和生长锥中的细胞器运动，伴随着生长锥前进的减慢。在一些生长锥中，层粘连蛋白引起初级神经突分支。在这些生长锥中，细胞器突出到新生的分支中。在分支，可溶性层粘连蛋白增加细胞器的运动在生长锥和轴的远端部分。这些结果表明，层粘连蛋白抑制初级神经突起的伸长，但促进分支和分支的伸长，所有这些似乎都与细胞器运输密切相关。© 2005 Wiley利斯公司
Laminin, an extracellular matrix molecule, is known to promote neurite growth. In the present study, the effects of soluble laminin on organelle transport and their relation to neurite growth were investigated in cultured dissociated mouse dorsal root ganglion (DRG) neurons. Laminin added into the extracellular medium was deposited on the surface of DRG neurons. DRG neurons incubated with soluble laminin exhibited branched, long, and thin neurites. Time‐lapse study demonstrated that many small‐diameter branches were newly formed after the addition of laminin. Thus, the growths of large‐diameter primary neuritis, arising from cell bodies and branches extended from growth cones of primary neuritis, were analyzed separately. Laminin decreased the growth rate of primary neurites but increased that of branches. In primary neurites, acute addition of laminin rapidly decreased organelle movement in the neurite shaft and growth cone, accompanied by slowing of the growth cone advance. Branching of primary neurites occurred in response to laminin in some growth cones. In these growth cones, organelles protruded into nascent branches. In branches, soluble laminin increased organelle movement in the growth cone and the distal portion of the shaft. These results suggest that laminin inhibits the elongation of primary neurites but promotes branching and elongation of branches, all of which seem to be closely related to organelle transport. © 2005 Wiley‐Liss, Inc.