26S rDNA-Based Phylogenetic Investigation of Japanese Cattle-Associated Prototheca zopfiii Isolates
26S rDNA-Based Phylogenetic Investigation of Japanese Cattle-Associated Prototheca zopfiii Isolates
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DOI：
10.1292/jvms.09-0115
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发表时间：
2010-01-01
影响因子：
1.2
通讯作者：
Kamata, Hiroshi
中科院分区：
文献类型：
--
作者：
Kishimoto, Yuji;Kano, Rui;Kamata, Hiroshi
本研究旨在探讨26S rDNA部分序列用于佐氏毕赤酵母基因型分析的可行性，以及佐氏毕赤酵母特异基因型与临床型乳腺炎的关系。系统发育分析表明，所有基因型2的分离株聚为一簇的模式菌株的佐氏疟原虫。因此，日本的所有乳腺炎分离株均被鉴定为佐氏疟原虫基因型2，与基因型I分离株的聚类无关。在一个临床病例中，粪便分离物不能通过基于18S rDNA的基因型特异性PCR检测来鉴定，并且通过26S rDNA分析似乎与布拉氏疟原虫的模式菌株属于同一簇。该分离株是日本首次分离。
The study has been conducted to assess the applicability of partial 26S ribosomal DNA sequence for the phylogenic analysis of P.zopfii and to arrive relationships between specific genotype of P.zopfii and clinical mastitis. The phylogenetic analysis indicated that all genotype 2 isolates were grouped into the Cluster of type strain of P.zopfii. Thus, all mastitis isolates in Japan were identified as P.zopfii genotype 2 and were independent of the cluster of the genotype I isolates. In one clinical case, a fecal isolate could not be identified by the 18S rDNA-based genotype specific PCR-assay and appeared to belong to a same cluster with the type strain of P. blaschkeae by 26S rDNA analysis. The isolate was the first isolation in Japan.