A Three-Dimensional Primary Cortical Culture System Compatible with Transgenic Disease Models, Virally Mediated Fluorescence, and Live Microscopy.
A Three-Dimensional Primary Cortical Culture System Compatible with Transgenic Disease Models, Virally Mediated Fluorescence, and Live Microscopy.
复制标题
与转基因疾病模型、病毒介导的荧光和活体显微镜兼容的三维原代皮质培养系统。
DOI：
10.1007/978-1-0716-3287-1_12
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Borton,DavidA
中科院分区：
文献类型：
--
作者：
Brown,Sophie;Atherton,Elaina;Borton,DavidA
体外细胞培养模型可以提供高分辨率和高通量的细胞行为实验。然而，在体外培养方法往往无法完全重演复杂的细胞过程，涉及异质性神经细胞群和周围的神经微环境之间的协同作用。在这里，我们描述了一个三维的原代皮层细胞培养系统与活的共聚焦显微镜兼容的形成。
In vitro cell culture models can offer high-resolution and high-throughput experimentation of cellular behaviors. However, in vitro culture approaches often fail to fully recapitulate complex cell processes involving synergistic interactions between heterogeneous neural cell populations and the surrounding neural microenvironment. Here, we describe the formation of a three-dimensional primary cortical cell culture system compatible with live confocal microscopy.