SYNTHESIS OF MUMPS-VIRUS POLYPEPTIDES IN INFECTED VERO CELLS
SYNTHESIS OF MUMPS-VIRUS POLYPEPTIDES IN INFECTED VERO CELLS
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DOI：
10.1016/0042-6822(82)90102-7
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发表时间：
1982-01-01
期刊：
影响因子：
3.7
通讯作者：
COMPANS, RW
中科院分区：
文献类型：
--
作者：
HERRLER, G;COMPANS, RW
腮腺炎病毒适应了非洲绿猴肾 Vero 细胞的生长，产生了高感染滴度的病毒。在 Vero 细胞中生长的纯化病毒体的结构多肽与之前描述的卵生腮腺炎病毒的结构多肽相似：L (200K)、HN (79K)、NP (72K)、F1 (61K)、P (45K)、M (40K) 和 F2 (16K)。通过用放射性氨基酸前体进行脉冲标记来分析 Vero 细胞中病毒多肽的合成。感染后 6 小时 (h.p.i.)，核蛋白 (NP) 首次在细胞内检测到高于细胞蛋白背景。注射后 12 小时，除了糖蛋白 F1 和 F2 外，所有病毒多肽均被观察到，它们源自称为 F0 (74K) 的前体。在受感染的细胞中也检测到了纯化病毒颗粒中不存在的两种低分子量多肽。它们被指定为 pI (28K) 和 pII (19K)。肽图分析显示，这 2 种多肽具有相同的氨基酸序列区域，并且它们也与结构蛋白 P 相关。在感染腮腺炎病毒的多种细胞类型（Vero、鸡胚成纤维细胞、Madin-Darby 牛肾 MDBK 细胞）中发现了多肽 pI 和 pII。用其他副粘病毒（猿猴病毒5和仙台病毒）感染Vero细胞并没有诱导与PI和PII相当的蛋白质的合成；在腮腺炎病毒感染的细胞中，没有检测到仙台病毒非结构C蛋白的对应物。脉冲追踪实验表明 pI 和 pII 可能不是通过蛋白水解裂解衍生自 P。
Mumps virus was adapted to growth in African green monkey kidney Vero cells, which yielded virus of high infectivity titers. The structural polypeptides of purified virions grown in Vero cells were similar to those described previously for egg-grown mumps virus: L (200K), HN (79K), NP (72K), F1 (61K), P (45K), M (40K) and F2 (16K). The synthesis of viral polypeptides in Vero cells was analyzed by pulse labeling with radioactive amino acid precursors. The nucleoprotein (NP) was the first to be detected intracellularly above the cellular protein background at 6 hours post-infection (h.p.i.). By 12 h.p.i., all viral polypeptides were observed except for the glycoproteins F1 and F2, which are derived from a precursor designated F0 (74K). Two low-MW polypeptides not present in purified virions were also detected in infected cells. They are designated pI (28K) and pII (19K). Peptide mapping revealed that these 2 polypeptides share regions of their amino acid sequence and that they are also related to the structural protein P. Polypeptides pI and pII were found in several cell types (Vero, chicken embryo fibroblast, Madin-Darby bovine kidney MDBK cells) infected with mumps virus. Infection of Vero cells with other paramyxoviruses (simian virus 5 and Sendai virus) did not induce the synthesis of proteins comparable to PI and PII; in mumps virus-infected cells no counterpart to the nonstructural C protein of Sendai virus was detected. Pulse-chase experiments suggest that pI and pII may not be derived from P by proteolytic cleavage.