Different roles for ApcD and ApcF in Synechococcus elongatus and Synechocystis sp. PCC 6803 phycobilisomes
Different roles for ApcD and ApcF in Synechococcus elongatus and Synechocystis sp. PCC 6803 phycobilisomes
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DOI：
10.1016/j.bbabio.2019.04.004
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发表时间：
2019-06-01
影响因子：
4.3
通讯作者：
Kirilovsky, Diana
中科院分区：
文献类型：
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作者：
Calzadilla, Pablo I.;Muzzopappa, Fernando;Kirilovsky, Diana
藻胆酶体，蓝藻的光收集复合体，是一个巨大的藻胆蛋白，含有额外的膜复合体，由杆状辐射的核心形成。藻胆体已经进化到能够有效地吸收太阳能量，并通过藻胆体的最后一个能量受体ApcD和ApcE将其传递给光系统。ApcF还通过与ApcE相互作用影响能量转移。本研究研究了ApcD和ApcF在长聚囊球菌和聚囊球菌PCC6803的能量转移和状态转变中的作用。我们的结果表明，这些蛋白在两个菌株的这两个过程中具有不同的作用。缺少ApcD和ApcF可以抑制聚囊藻的状态转变，而在长形螺中则没有。此外，缺ApcF会减少向两个光系统的能量转移，而缺ApcD只会改变向光系统I的能量转移。因此，基于一种蓝藻菌株的结果得出的结论不能系统地转移到其他菌株，并且需要重新考虑藻胆异构体在状态转变中的假定作用。
The phycobilisome, the cyanobacterial light harvesting complex, is a huge phycobiliprotein containing extra membrane complex, formed by a core from which rods radiate. The phycobilisome has evolved to efficiently absorb sun energy and transfer it to the photosystems via the last energy acceptors of the phycobilisome, ApcD and ApcE. ApcF also affects energy transfer by interacting with ApcE. In this work we studied the role of ApcD and ApcF in energy transfer and state transitions in Synechococcus elongatus and Synechocystis PCC6803. Our results demonstrate that these proteins have different roles in both processes in the two strains. The lack of ApcD and ApcF inhibits state transitions in Synechocystis but not in S. elongatus. In addition, lack of ApcF decreases energy transfer to both photosystems only in Synechocystis, while the lack of ApcD alters energy transfer to photosystem I only in S. elongatus. Thus, conclusions based on results obtained in one cyanobacterial strain cannot be systematically transferred to other strains and the putative role(s) of phycobilisomes in state transitions need to be reconsidered.