The Expression Characteristics of Transcription Factors Regulated by Exogenous ABA in Winter Wheat (Triticum aestivum) under Cold Stress
The Expression Characteristics of Transcription Factors Regulated by Exogenous ABA in Winter Wheat (Triticum aestivum) under Cold Stress
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冷胁迫下外源ABA调控冬小麦转录因子的表达特性
DOI：
10.1134/s1021443718060134
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发表时间：
2018-11-01
影响因子：
1.4
通讯作者：
Cang, J.
中科院分区：
文献类型：
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作者：
Lv, Y.;Song, C. H.;Cang, J.
东农冬麦1号（Dn1）是小麦（Triticum aestivum L.）的一个品种，田间耐30℃，恢复率85%。ABA可以增强植物的抗性。为了揭示外源ABA （exo-ABA）处理（对照：非ABA处理）后Dn1的抗寒机制，选择并检测了一些转录因子（TFs）。幼苗在三叶期施用外源aba，然后分别在5℃、0℃、-10℃和25℃的田间取样。选择6个TF基因（TabZIP1、TaWABI5、TaMYB1、TaMYB80、TaNAC2和TaWRKY80），检测其在不同低温下的表达情况，结果表明：在非aba处理的耕作节点中，6个TF基因的表达均在10℃时达到峰值；外源aba处理后，5个TF基因（TabZIP1、TaWABI5、TaMYB80、TaNAC2和TaWRKY80）在25℃时表达量达到峰值，其中TabZIP1在5个TF中表达量最高。为了进一步明确TabZIP1的生物学功能，利用生物信息学软件或在线程序对其理化性质、蛋白质二级结构和进化树进行了分析和预测；利用激光扫描共聚焦显微镜（LSCM）观察到TabZIP1定位于烟草（Nicotiana tabacum）的细胞核中。本研究初步揭示了外源aba处理后，Dn1基因中部分TFs表达上调以响应冷胁迫，这表明在TFs的帮助下，可以转录出严重的冷应答基因，为揭示冬小麦的冷应答机制提供了新的理论依据。
Dongnong Dongmai1 (Dn1)' is a cultivar of wheat (Triticum aestivum L.) which may endure30 degrees C in field with a reviving rate of 85%. ABA may enhance plant resistance. Aiming to reveal Dn1 coldresistant mechanism after exogenous ABA (exo-ABA) treated (control: non-ABA treated), some transcription factors (TFs) were selected and detected. Seedlings were prayed with exo-ABA at three-leaf stage, subsequently their tilling nodes were respectively sampled when it was at 5, 0,-10 and-25 degrees C in field. Six TF genes (TabZIP1, TaWABI5, TaMYB1, TaMYB80, TaNAC2 and TaWRKY80) were selected and their expression at different low temperature was detected, the results showed that: in non-ABA treated tilling nodes, the expression of six TF genes all reached their peak level at-10 degrees C; in exo-ABA treated tilling nodes, the expression of five TF genes (TabZIP1, TaWABI5, TaMYB80, TaNAC2 and TaWRKY80) reached their peak level at-25 degrees C, and particularly the expression level of TabZIP1 was the highest in five TFs. In order to further defined the biological function of TabZIP1, the physical and chemical properties, protein secondary structure and the evolutionary tree was analyzed and predicted by some bioinformatics software or online program; TabZIP1 was localized in nuclear of tobacco (Nicotiana tabacum), which was observed by laser scanning confocal microscope (LSCM). This study initially exposed some TFs in Dn1 were upregulated to respond to the coldstress after exo-ABA treated, which indicated that a serious cold-responsive genes could be transcripted with the TFs assistance, and the results provided new theoretical basis to reveal the cold-reponsive mechanism of winter wheat.