Genetic Features of MCR-1-Producing Colistin-Resistant Escherichia coli Isolates in South Africa
Genetic Features of MCR-1-Producing Colistin-Resistant Escherichia coli Isolates in South Africa
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DOI：
10.1128/aac.00444-16
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发表时间：
2016-07-01
影响因子：
4.9
通讯作者：
Nordmann, Patrice
中科院分区：
文献类型：
--
作者：
Poirel, Laurent;Kieffer, Nicolas;Nordmann, Patrice
从南非的住院患者和社区患者中回收了一系列粘菌素耐药大肠杆菌临床分离株。7个克隆无关的分离株携带位于不同质粒骨架上的mcr-1基因。对两种不同的质粒进行了完全测序，并鉴定了定义mcr-1盒的相同的2，600 bp长DNA序列。对负责mcr-1表达的启动子序列进行了表征，该启动子序列是根据mcr-1的+1转录起始位点的精确鉴定而推导出的。
A series of colistin-resistant Escherichia coli clinical isolates was recovered from hospitalized and community patients in South Africa. Seven clonally unrelated isolates harbored the mcr-1 gene located on different plasmid backbones. Two distinct plasmids were fully sequenced, and identical 2,600-bp-long DNA sequences defining a mcr-1 cassette were identified. Promoter sequences responsible for the expression of mcr-1, deduced from the precise identification of the +1 transcription start site for mcr-1, were characterized.