Comparison of hepatitis C virus genotyping by 5′ noncoding region- and core-based reverse transcriptase PCR assay with sequencing and use of the assay for determining subtype distribution in India
Comparison of hepatitis C virus genotyping by 5′ noncoding region- and core-based reverse transcriptase PCR assay with sequencing and use of the assay for determining subtype distribution in India
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DOI：
10.1128/jcm.41.11.5240-5244.2003
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发表时间：
2003-11-01
影响因子：
9.4
通讯作者：
Arankalle, VA
中科院分区：
文献类型：
--
作者：
Lole, KS;Jha, JA;Arankalle, VA
对代表印度北部、南部、东部和西部的 149 份丙型肝炎病毒 (HCV) RNA 阳性慢性携带者样本的 5' 非编码区 (5'NCR) 序列进行系统发育分析表明，3 型和 1 型是印度流行的主要基因型，总体患病率分别为 53.69% 和 38.25%。 4 型病毒（6.04%）仅在印度南部发现。对上述 51 个分离株的核心区域进行序列分析，使我们能够将它们进一步分为亚型：1b（分离株数量 [n] = 10）、1a（n = 6）、3a（n = 9）、3g（n = 14）、3f（n = 1）和 4d（n = 3）。首次鉴定出三种新亚型，并指定为 3i (n = 5)、3j (n = 2) 和 61 (n = 1)。对 5'NCR 进行测序可以区分 HCV 类型，而通过核心区域的序列分析可以进行亚型级别的分类。
Phylogenetic analysis of the sequences of the 5' noncoding regions (5'NCR) of 149 samples from hepatitis C virus (HCV) RNA-positive chronic carriers representing northern, southern, eastern, and western India showed that type 3 and type 1 are the predominant genotypes circulating in India, with an overall prevalence of 53.69 and 38.25%, respectively. Type 4 viruses (6.04%) were seen only in southern India. Sequence analysis of the core region of 51 of the above isolates enabled us to classify them further into subtypes as 1b (number of isolates [n] = 10), 1a (n = 6), 3a (n = 9), 3g (n = 14), 3f (n = 1), and 4d (n = 3). Three new subtypes were identified for the first time and designated as 3i (n = 5), 3j (n = 2), and 61 (n = 1). Sequencing the 5'NCR could differentiate HCV types, whereas classification at the level of subtype was possible with sequence analysis of the core region.