Targeting DNA Double-Strand Breaks with TAL Effector Nucleases
Targeting DNA Double-Strand Breaks with TAL Effector Nucleases
复制标题
DOI：
10.1534/genetics.110.120717
复制
发表时间：
2010-10-01
期刊：
影响因子：
3.3
通讯作者：
Voytas, Daniel F.
中科院分区：
文献类型：
--
作者：
Christian, Michelle;Cermak, Tomas;Voytas, Daniel F.
在体内切割特异性DNA序列的工程化核酸酶是用于靶向诱变的有价值的试剂。在这里，我们报告了一类新的序列特异性核酸酶，通过融合转录激活因子样效应子（TALE）的FokI内切核酸酶的催化结构域。天然和定制的TALE-核酸酶融合体都将DNA双链断裂引导至特定的靶位点。
Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.