Induction of cytochrome P-450s and expression of liver-specific genes in rat primary hepatocytes cultured on different extracellular matrices.
Induction of cytochrome P-450s and expression of liver-specific genes in rat primary hepatocytes cultured on different extracellular matrices.
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在不同细胞外基质上培养的大鼠原代肝细胞中细胞色素 P-450 的诱导和肝脏特异性基因的表达。
DOI：
10.1271/bbb.58.1514
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
A. Yoshida
中科院分区：
文献类型：
--
作者：
N. Matsushita;H. Oda;K. Kobayashi;T. Akaike;A. Yoshida
新鲜分离的肝细胞在由 EHS 肿瘤、聚 N-对乙烯基苄基-D-内酰胺 (PVLA) 和 I 型胶原 (TIC) 制备的 EHS 凝胶上培养。 EHS-gel 上的肝细胞呈球形，并且更强烈地维持了在 PVLA 和 TIC 上丢失的细胞色素 P-450 基因的诱导表达。此外，肝脏特异性基因的表达在 EHS 凝胶上保持在最高水平，然后在 PVLA 上高于 TIC。
Freshly isolated hepatocytes were cultured on an EHS-gel prepared from EHS-tumor, poly-N-p-vinylbenzyl-D-lactonamide (PVLA), and type I collagen (TIC). Hepatocytes on EHS-gel showed a spherical shape and much more strongly maintained the inducible expression of cytochrome P-450 genes which were lost on PVLA and TIC. Further, the expression of liver-specific genes were maintained on EHS gel at the highest level, and then higher on PVLA than TIC.