Role of heme oxygenase-1 in human placenta on iron supply to fetus
Role of heme oxygenase-1 in human placenta on iron supply to fetus
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DOI：
10.1016/j.placenta.2020.09.065
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发表时间：
2021-01-01
期刊：
影响因子：
3.8
通讯作者：
Akagi, Reiko
中科院分区：
文献类型：
--
作者：
Inoue, Rikako;Irie, Yasuyuki;Akagi, Reiko
简介：迄今为止，关于铁如何通过胎盘从母亲提供给胎儿的细节仍然不清楚。近年来，越来越多的研究表明血红素氧合酶（HO）-1是血红素降解途径中的限速酶，可能参与铁的有效再利用。本研究检测了HO-1在不同妊娠时期人胎盘绒毛组织中的分布和基因表达情况。方法：采用经知情同意的38例胎盘组织，通过组织学检查，观察HO-1蛋白在胎盘绒毛组织中的分布和基因表达的时间变化。RT-PCR检测HO-1、转铁蛋白受体-1和ferroportin 1的表达。结果：免疫组化结果显示HO-1蛋白在整个妊娠过程中只表达于滋养细胞。在妊娠早期的流产胎盘中，ho-1 mRNA水平显著高于正常。在妊娠早期和中期出现死胎（流产）的胎盘中，铁生成基因ho-1与铁排泄基因fpn-1的比例明显高于正常。这些结果表明，HO-1的各种生理功能的作用是不断变化的整个pregnancy.Discussion：这些研究结果表明，HO-1在胎盘铁供应系统中发挥重要作用，在中期妊娠，以支持胎儿的发育。
Introduction: To date, details on how iron is supplied from the mother to the fetus through the placenta have remained unclear. Recently, increasing evidence has shown that heme oxygenase (HO)-1, which is an inducible isoform of the rate-limiting enzyme in the heme degradation pathway, may be involved in the effective reutilization of iron. In this study, we examined the distribution and gene expression of HO-1 in the villous tissue of human placenta at various periods of pregnancy.Methods: Using the placenta of 38 samples for which consent was obtained, chronological changes in the localization of HO-1 protein were examined by histological examination. RT-PCR was also performed to examine the expression of HO-1, transferrin receptor-1, and ferroportin 1. Ferric iron in the tissues was analyzed by Prussian blue staining.Results: Immunohistochemical studies showed that HO-1 protein was exclusively expressed in trophoblastic cells throughout gestation. In the miscarriage placenta in the first trimester, ho-1 mRNA levels were significantly higher than normal. Placenta with fetal death (miscarriage) in the first and second trimester indicate significantly higher ratio of ho-1 gene for iron production to the fpn-1 gene for iron excretion than normal. These suggest that the role of HO-1 with various physiological functions is changing throughout pregnancy.Discussion: These findings suggest that HO-1 in placenta plays an important role in iron supplying system in the second trimester to support fetal development.