Photodynamic therapy of pigmented choroidal melanomas of greater than 3-mm thickness.
Photodynamic therapy of pigmented choroidal melanomas of greater than 3-mm thickness.
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厚度大于 3 毫米的色素性脉络膜黑色素瘤的光动力疗法。
DOI：
10.1016/s0161-6420(96)30391-6
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发表时间：
1996
期刊：
影响因子：
13.7
通讯作者：
Young,LH
中科院分区：
文献类型：
--
作者：
Kim,RY;Hu,LK;Foster,BS;Gragoudas,ES;Young,LH
目的：研究苯并卟啉衍生物维替泊芬（verteporfin）脂质体光动力疗法对厚度≤ 3 mm的实验性色素性脉络膜黑色素瘤的杀伤作用。方法：在32只新西兰白化病兔眼建立色素性脉络膜肿瘤模型。每天注射环孢霉素治疗动物，并通过系列眼底检查和超声检查跟踪肿瘤生长。当肿瘤厚度超过3 mm（肿瘤高度范围为3.1-4.6 mm）时，作者静脉内给予苯并卟啉衍生物（1 mg/kg），并通过氩泵浦染料激光器以60 - 120 J/cm 2的不同总光剂量在692 nm处照射肿瘤。对照动物仅用光或苯并卟啉衍生物处理。结果：苯并卟啉衍生物和≤80 J/cm 2的光通量治疗后，所有动物均显示肿瘤完全停止。相比之下，两个对照组在所有动物中均显示出连续的肿瘤生长，到3周时肿瘤填充了大部分玻璃体腔。治疗后立即用染料加光治疗的肿瘤的组织学检查结果显示显著的血管闭合。在仅用光或染料处理的对照眼中未观察到血管变化。检查结果的眼睛，显示肿瘤消退后，4周的随访期显示肿瘤坏死和广泛浸润的单核细胞和色素负载的巨噬细胞在肿瘤location.Conclusion：这些数据表明，光动力疗法可能有一个角色，在管理色素性脉络膜黑色素瘤。
Purpose:The purpose of the study is to determine the effect of photodynamic therapy in the destruction of experimental pigmented choroidal melanomas ≤ 3 mm in thickness using a liposomal preparation of benzoporphyrin derivative, verteporfin.Methods:Pigmented choroidal tumors were established in 32 New Zealand albino rabbit eyes. Animals were treated with daily injections of cyclosporine, and tumor growth was followed by serial fundus examinations and ultrasonography. When a tumor exceeded 3 mm in thickness (tumor height ranged from 3.1–4.6 mm), the authors administered benzoporphyrin derivative intravenously (1 mg/kg) and irradiated the tumor at 692nm through an argon-pumped dye laser at different total light doses ranging from 60 to 120 J/cm2. Control animals were treated with light or benzoporphyrin derivative only. Each animal then was followed-up for 4 to 6 weeks by fundus photography, fluorescein angiography, and ultrasonography.Results:All animals treated with benzoporphyrin derivative and light at fluences of ≤80 J/cm2showed complete tumor arrest. In contrast, both control groups showed continuous tumor growth in all animals with tumors filling most of the vitreous cavity by 3 weeks. Histologic examination results of tumors treated with dye plus light immediately after treatment showed prominent vascular closure. No vascular changes were noted in the control eye treated with light or dye alone. Examination results of the eyes that showed tumor regression after a 4week follow-up period showed tumor necrosis and extensive infiltration of mononuclear cells and pigment-laden macrophages at the tumor site.Conclusion:These data suggest that photodynamic therapy may have a role in the management of pigmented choroidal melanomas.