A panel of new microsatellite loci for genetic studies of antarctic fur seals and other otariids
A panel of new microsatellite loci for genetic studies of antarctic fur seals and other otariids
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用于南极海狗和其他小动物遗传研究的一组新微卫星基因座
DOI：
10.1007/s10592-008-9669-z
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发表时间：
2009
影响因子：
2.2
通讯作者：
J. Hoffman
中科院分区：
文献类型：
--
作者：
J. Hoffman
在南极毛皮海豹Arctocephalus gazella中开发了9个dinucelotide微卫星位点。每个基因座具有4和9个等位基因之间的20个个体的样本中采样从鸟岛，南格鲁吉亚，预期的杂合性范围从0.52到0.84。所有位点均符合Hardy-Weinberg平衡，未发现基因型不平衡。此外，所有的基因座成功地交叉扩增在南美毛皮sealArctocephalus australis，新西兰毛皮sealArctocephalus forsteriandSteller的海狮Eumetopias jubatus，和六个也产生了产品在更遥远的相关港口sealPhoca vitulina和海象Odobenus rosmarus。这些基因座应证明是有用的南极海狗和其他重要的otariid物种的群体遗传学研究。
Nine dinucelotide microsatellite loci were developed in the Antarctic fur sealArctocephalus gazella. Each locus possessed between 4 and 9 alleles in a sample of twenty individuals sampled from Bird Island, South Georgia, and expected heterozygosity ranged from 0.52 to 0.84. All but one of the loci conformed to Hardy–Weinberg equilibrium and no evidence was found for genotypic disequilibrium. Additionally, all of the loci successfully cross-amplified in the South American fur sealArctocephalus australis, the New Zealand fur sealArctocephalus forsteriand the Steller’s sea lionEumetopias jubatus, and six also yielded products in the more distantly related harbour sealPhoca vitulinaand walrusOdobenus rosmarus. These loci should prove useful for studies of the population genetics of Antarctic fur seals and other important otariid species.