Environmental monitoring for biological threat agents using the Autonomous Pathogen Detection System with multiplexed polymerase chain reaction
Environmental monitoring for biological threat agents using the Autonomous Pathogen Detection System with multiplexed polymerase chain reaction
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DOI：
10.1021/ac801125x
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发表时间：
2008-10-01
影响因子：
7.4
通讯作者：
Dzenitis, John M.
中科院分区：
文献类型：
--
作者：
Regan, John F.;Makarewicz, Anthony J.;Dzenitis, John M.
我们开发并实地测试了一种现已投入使用的民用生物防御能力，这种能力持续监测高风险地点的空气中是否有生物威胁剂。这种独立的仪器被称为自主病原体检测系统（APDS），它收集并选择性地将空气中的颗粒物浓缩到液体样品中，并使用多重PCR扩增结合微球阵列检测来分析样品。在实验室测试期间，我们通过向液体样品流中加入活孢子和细胞、珠粒破碎的裂解物和纯化的DNA提取物，评估了APDS仪器对炭疽杆菌和鼠疫耶尔森菌的响应。还将APDS结果与手动实时PCR方法进行了比较。在一个轨道交通地铁站连续运行74天的现场数据，证明了APDS的特异性和可靠性。美国国土安全部最近选择了本文报道的APDS作为其BioWatch反恐计划的第一个自主探测器组件。这种先进的现场部署监视能力现在可以在人工过滤器收集和分析时间的十分之一内生成可操作的数据。
We have developed and field-tested a now operational civilian biodefense capability that continuously monitors the air in high-risk locations for biological threat agents. This stand-alone instrument, called the Autonomous Pathogen Detection System (APDS), collects and selectively concentrates particles from the air into liquid samples and analyzes the samples using multiplexed PCR amplification coupled with microsphere array detection. During laboratory testing, we evaluated the APDS instrument's response to Bacillus anthracis and Yersinia pestis by spiking the liquid sample stream with viable spores and cells, bead-beaten lysates, and purified DNA extracts. APDS results were also compared to a manual real-time PCR method. Field data acquired during 74 days of continuous operation at a mass-transit subway station are presented to demonstrate the specificity and reliability of the APDS. The U.S. Department of Homeland Security recently selected the APDS reported herein as the first autonomous detector component of their BioWatch anti-terrorism program. This sophisticated field-deployed surveillance capability now generates actionable data in one-tenth the time of manual filter collection and analysis.