COMPARISON OF OXIDATIVE-METABOLISM INVITRO IN ENDOTHELIAL-CELLS FROM DIFFERENT SPECIES AND VESSELS
COMPARISON OF OXIDATIVE-METABOLISM INVITRO IN ENDOTHELIAL-CELLS FROM DIFFERENT SPECIES AND VESSELS
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DOI：
10.1002/jcp.1041320323
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发表时间：
1987-09-01
影响因子：
5.6
通讯作者：
RISBERG, B
中科院分区：
文献类型：
--
作者：
KJELLSTROM, BT;ORTENWALL, P;RISBERG, B
比较了人脐带静脉、大鼠肺动脉和牛主动脉内皮细胞融合培养的耗氧量。采用氧合血红蛋白作为供氧指标和呼吸指标的微呼吸测定法。人和小鼠细胞呼吸频率相等（2.0倍）。10-6和2.2倍。10 - 6 .mu。l O2/细胞/小时)，而内皮细胞的呼吸速率显著低于细胞间的呼吸速率(0.4。10 - 6 .mu。l O2/cell/h) （P < 0.001）。
Oxygen consumption was compared in confluent cultures of endothelial cells from human umbilical cord veins, rat pulmonary arteries, and bovine aortas. A microrespirometric method utilizing oxygenated hemoglobin as oxygen supply and indicator of respiration was used. Respiratory rate was equal in human and murine cells (2.0 .times. 10-6 and 2.2 .times. 10-6 .mu.l O2/cell/hour respectively), compared on a cell-to-cell basis, while respiratory rate of the bovine endothelium was significantly lower (0.4 .times. 10-6 .mu.l O2/cell/hour) (P < .001).