Expression of the Na+-D-glucose cotransporter SGLT1 in neurons
Expression of the Na+-D-glucose cotransporter SGLT1 in neurons
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DOI：
10.1046/j.1471-4159.1997.69010084.x
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发表时间：
1997-07-01
影响因子：
4.7
通讯作者：
Koepsell, H
中科院分区：
文献类型：
--
作者：
Poppe, R;Karbach, U;Koepsell, H
在兔、猪和人脑中，证明了高亲和力Na+-D-葡萄糖协同转运蛋白SGLT 1和改变SGLT 1活性的蛋白质RS 1的表达。原位杂交结果显示，SGLT 1和RSI在大脑皮层和海马的锥体细胞以及小脑的浦肯野细胞中转录。在猪脑神经元中，通过突触体膜的Western印迹和免疫组织化学显示SGLT 1蛋白，其显示SGLT 1在锥体细胞和浦肯野细胞中。为了检测神经元中的SGLT 1是否可能在D-葡萄糖消耗增加期间被激活，在大鼠脑中诱导癫痫发作，并通过放射自显影术分析SGLT 1 {[C-14]甲基-α-D-吡喃葡萄糖苷（[C-14]AMG）}和Na+非依赖性转运蛋白{2-脱氧-D-[C-14]葡萄糖（[C-14]2-DG）}的特定非代谢底物的摄取。癫痫发作时，病灶内AMG和2-DG摄取增加。癫痫发作后2小时内，病灶中的2-DG摄取恢复正常。相反，1天后病灶区的AMG摄取仍增加。数据显示，高亲和力Na+-D-葡萄糖协同转运蛋白SGLT 1在神经元中表达，并且可以上调。
In brains of the rabbit, pig, and human, expression of the high-affinity Na+-D-glucose cotransporter SGLT1 and of the protein RS1, which alters the activity of SGLT1, was demonstrated. In situ hybridization showed that SGLT1 and RSI are transcribed in pyramidal cells of brain cortex and hippocampus and in Purkinje cells of cerebellum. In neurons of pig brain SGLT1 protein was demonstrated by western blotting with synaptosomal membranes and by immunohistochemistry, which showed SGLT1 in pyramidal and Purkinje cells. To test whether SGLT1 in neurons may be activated during increased D-glucose consumption, an epileptic seizure was induced in rat brain, and the uptake of specific nonmetabolized substrates of SGLT1 {[C-14] methyl-alpha-D-glucopyranoside ([C-14]AMG)} and of Na+-independent transporters {2-deoxy-D-[C-14] glucose ([C-14]2-DG)} was analyzed by autoradiography. During the seizure the uptake of AMG and 2-DG was increased in the focus. Within two hours after the seizure 2-DG uptake in the focus returned to normal. In contrast, the AMG uptake in the focus area was still increased 1 day later. The data show that the high-affinity Na+-D-glucose cotransporter SGLT1 is expressed in neurons and can be up-regulated.