Optimized extraction of polysaccharides from Taxus chinensis var. mairei fruits and its antitumor activity
Optimized extraction of polysaccharides from Taxus chinensis var. mairei fruits and its antitumor activity
复制标题
红豆杉多糖的优化提取
DOI：
10.1016/j.ijbiomac.2015.01.043
复制
发表时间：
2015-04-01
影响因子：
8.2
通讯作者：
Lu, Zhicheng
中科院分区：
文献类型：
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作者：
Zhao, Chunjian;Li, Zhao;Lu, Zhicheng
超声波/微波协同萃取法（UMAE）是一种有效的红豆杉多糖提取方法。梨果实（TCFPs）。在这项研究中，我们使用响应面法，以确定最佳的TCFPs提取条件。结果表明，最佳工艺条件为：料液比33 mL/g，提取时间10 min，微波功率560 W，超声功率50 W。在优化条件下，通过UMAE获得的TCFPs产率为4.33 +/-0.15%，与传统的加热回流提取（HRE）相比，增加了1.79倍。此外，UMAE中使用的提取时间比HRE所需的时间短：10分钟对90分钟。因此，UMAE是一种快速有效的TCFPs提取方法。同时研究了TCFPs对S180肿瘤生长的抑制作用。TCFPs的抑瘤率为76.33%，具有一定的抑瘤作用。器官重量分析表明，与阳性对照组相比，TCFPs对肝、肾、脾、心或肺无毒性。因此，TCFPs显示出抗肿瘤活性，无器官毒性。(C)2015 Elsevier B.V.版权所有。
The simultaneous ultrasonic/microwave-assisted extraction (UMAE) method is potentially useful for the extraction of polysaccharides from Taxus chinensis var. mairei fruits (TCFPs). In this study, we used a response surface methodology to identify optimal TCFPs extraction conditions. Optimal parameters were determined as follows: a liquid to raw material ratio of 33 mL/g, an extraction time of 10 min, a microwave power level of 560 W, and a fixed ultrasonic power of 50 W. Under the optimized conditions, TCFPs yields obtained by UMAE were 4.33 +/- 0.15%, a 1.79-fold increase compared with conventional heating reflux extraction (HRE). In addition, the extraction time used in UMAE was shorter than that required for HRE: 10 versus 90 min. UMAE is therefore a rapid and efficient method for the extraction of TCFPs. The inhibitory effect of TCFPs on S180 tumor growth in vivo was also studied. The tumor inhibition rate of TCFPs was 76.33%, indicating a tumor-inhibiting effect. Analysis of organ weights demonstrated that TCFPs exhibited no toxicity to liver, kidney, spleen, heart, or lung relative to a positive control group. TCFPs thus show antitumor activity with no organ toxicity. (C) 2015 Elsevier B.V. All rights reserved.