Nanofluidic Cells with Controlled Path length and Liquid Flow for Rapid, High-Resolution In Situ Imaging with Electrons
Nanofluidic Cells with Controlled Path length and Liquid Flow for Rapid, High-Resolution In Situ Imaging with Electrons
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DOI：
10.1021/jz401067k
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发表时间：
2013-07-18
影响因子：
5.7
通讯作者：
Miller, R. J. Dwayne
中科院分区：
文献类型：
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作者：
Mueller, C.;Harb, M.;Miller, R. J. Dwayne
使用电子探针原位成像的溶液相系统一直是一个长期持有的目标，主要是由原子分辨率的分子结构动力学相关的化学和生物学的前景。在这里，我们提出了一个纳米流体样品池与主动反馈，以保持稳定的流动条件，从45 nm到几个100 nm的路径长度变化，在一个可用的观察面积为50 × 50 μ m。使用这个概念，我们展示了纳米分辨率成像弱散射聚合物和高散射纳米粒子并排与传统的透射显微镜。流动液体的能力允许控制样品含量和即时样品交换，开辟了高通量电子显微镜的领域。的nanofluidic细胞设计的特点是简单，可靠，操作与外部液体样品控制成像（扫描）传输模式，并持有很大的希望，在飞秒电子衍射研究的溶液相反应动力学的倒易空间成像。
The use of electron probes for in situ imaging of solution phase systems has been a long held objective, largely driven by the prospect of atomic resolution of molecular structural dynamics relevant to chemistry and biology. Here, we present a nanofluidic sample cell with active feedback to maintain stable flow conditions for pathlengths varying from 45 nm to several 100 nm, over a useable viewing area of 50 X 50 mu m. Using this concept, we demonstrate nanometer resolution for imaging weakly scattering polymer and highly scattering nanoparticles side by side with a conventional transmission microscope. The ability to flow liquids allows control over sample content and on-the-fly sample exchange, opening up the field of high-throughput electron microscopy. The nanofluidic cell design is distinguished by straightforward, reliable, operation with external liquid specimen control for imaging in (scanning) transmission mode and holds great promise for reciprocal space imaging in femtosecond electron diffraction studies of solution phase reaction dynamics.