Taishan Pinus Massoniana pollen polysaccharide inhibits the replication of acute tumorigenic ALV-J and its associated tumor growth
Taishan Pinus Massoniana pollen polysaccharide inhibits the replication of acute tumorigenic ALV-J and its associated tumor growth
复制标题
泰山马尾松花粉多糖抑制急性致瘤ALV-J复制及其相关肿瘤生长
DOI：
10.1016/j.vetmic.2019.07.028
复制
发表时间：
2019-09-01
影响因子：
3.3
通讯作者：
Zhu, Ruiliang
中科院分区：
文献类型：
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作者：
Wang, Qiuju;Miao, Yongqiang;Zhu, Ruiliang
J亚群禽白血病病毒（ALV-J）给养禽业造成了巨大的经济损失。近年来，由含有v-fps癌基因的ALV-J引起的纤维肉瘤的势头越来越大，这给家禽业带来了新的挑战。为研究泰山马尾松花粉多糖（TPPPS）对J型ALV-J急性感染和肿瘤发生的抑制作用，建立了J型ALV-J福J株（SDAU 1005）的体内外抗病毒和抗肿瘤模型。体外实验结果表明，在吸附和预处理阶段，TPPPS以剂量依赖的方式显著抑制病毒复制。体内实验结果表明，TPPPS显著降低血浆和肿瘤组织中的病毒载量，以及抑制肿瘤生长。我们通过使用RNA-Seq技术进一步检查了转录组表达的差异。共鉴定出560个差异表达基因，其中上调基因329个，下调基因231个。上调基因主要为免疫相关基因，下调基因主要为肿瘤相关基因。基因本体（GO）术语富集包括免疫系统过程、免疫系统过程的正调节、免疫系统过程的调节、白细胞活化、细胞活化和蛋白质结合。京都基因和基因组百科全书（KEGG）通路分析显示，主要的免疫和肿瘤相关通路包括T细胞受体信号通路、嘌呤-细胞因子受体相互作用、自然杀伤细胞介导的细胞毒性、PI3 K-Akt信号通路、JAK-STAT信号通路、NF-κ B信号通路和Ras信号通路。本研究结果初步揭示了TPPPS在体内外抗病毒和抗肿瘤的作用机制。
Avian leukosis virus subgroup J (ALV-J) has resulted in considerable economic losses in the poultry industry. In recent years, fibrosarcoma induced by ALV-J, which contains the v-fps oncogene, has gained momentum, and this has brought about new challenges to the poultry industry. To study the inhibitory effects of Taishan Pinus Massoniana pollen polysaccharide (TPPPS) on acute ALV-J infection and tumor development, antiviral and antitumor models of the Fu-J (SDAU1005) strain of ALV-J were established in vitro and in vivo. The results of in vitro experiments showed that TPPPS significantly inhibited viral replication in a dose-dependent manner during adsorption and pretreatment stages. The results of in vivo experiments have shown that TPPPS significantly reduced the viral load in the plasma and tumor tissues, as well as inhibited tumor growth. We further examined the difference in transcriptome expression by using RNA-Seq technology. A total of 560 differentially expressed genes were identified that included 329 up-regulated genes and 231 down-regulated genes. The up-regulated genes were mainly immune-related genes, whereas the down-regulated genes were mainly tumor-regulated genes. Gene Ontology (GO) term enrichment included immune system processes, positive regulation of immune system processes, regulation of immune system processes, leukocyte activation, cell activation, and protein binding. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the main immune and tumor-related pathways included T-cell receptor signaling pathway, cytokine-cytokine receptor interactions, natural killer cell-mediated cytotoxicity, PI3K-Akt signaling pathway, JAK-STAT signaling pathway, NF-kappa B signaling pathway, and Ras signaling pathway. In summary, our results preliminarily point to the antiviral and antitumor mechanism of TPPPS in vivo and in vitro.