Label free fluorescence turn-on detection of polynucleotide kinase activity with a perylene probe
Label free fluorescence turn-on detection of polynucleotide kinase activity with a perylene probe
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使用苝探针进行多核苷酸激酶活性的无标记荧光开启检测
DOI：
10.1039/c2cc32644b
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发表时间：
2012-01-01
影响因子：
4.9
通讯作者：
Yu, Cong
中科院分区：
文献类型：
--
作者：
Jiao, Huping;Wang, Bin;Yu, Cong
单链寡核苷酸可以诱导聚乙二醇探针聚集，探针的单体荧光被有效猝灭。然而，当寡核苷酸被多核苷酸激酶5'-磷酸化时，它可以被lambda外切酶非常有效地降解，探针单体被释放，并检测到一个开启的荧光信号。
A single stranded oligonucleotide could induce aggregation of a perylene probe, the probe's monomer fluorescence was efficiently quenched. However, when the oligonucleotide was 5'-phosphorylated by polynucleotide kinase, it could be very efficiently degraded by lambda exonuclease, probe monomers were released, and a turn on fluorescence signal was detected.