Mechanism of apoptosis induced by apigenin in HepG2 human hepatoma cells: Involvement of reactive oxygen species generated by NADPH oxidase
Mechanism of apoptosis induced by apigenin in HepG2 human hepatoma cells: Involvement of reactive oxygen species generated by NADPH oxidase
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DOI：
10.1007/bf02980274
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发表时间：
2007-10-01
影响因子：
6.7
通讯作者：
Lee, Yong Soo
中科院分区：
文献类型：
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作者：
Choi, Soo Im;Jeong, Choon Sik;Lee, Yong Soo
虽然植物来源的黄酮类化合物已被报道具有抗癌活性，但这些作用的确切机制还不完全清楚。在这项研究中，我们研究了活性氧物种(ROS)在芹菜素(一种广泛存在于植物中的黄酮类化合物)诱导HepG2人肝癌细胞凋亡中的作用。芹菜素以剂量依赖的方式降低细胞存活率，并诱导细胞凋亡。此外，它还引起细胞内ROS水平的剂量相关升高。用不同的NADPH氧化酶抑制剂(二苯基碘、罗布宁、新喋呤)处理后，可显著抑制由芹菜素诱导的ROS的产生和诱导细胞凋亡。这些结果提示，通过激活NADPH氧化酶产生的ROS可能在芹菜素诱导的HepG2细胞凋亡中起重要作用。这些结果进一步表明，芹菜素可能对人肝癌的治疗有价值。
Although plant-derived flavonoids have been reported to have anti-cancer activities, the exact mechanism of these actions is not completely understood. In this study we investigated the role for reactive oxygen species (ROS) as a mediator of the apoptosis induced by apigenin, a widespread flavonoid in plant, in HepG2 human hepatoma cells. Apigenin reduced cell viability, and induced apoptotic cell death in a dose-dependent manner. In addition, it evoked a dose-related elevation of intracellular ROS level. Treatment with various inhibitors of the NADPH oxidase (diphenylene iodonium, apocynin, neopterine) significantly blunted both the generation of ROS and induction of apoptosis induced by apigenin. These results suggest that ROS generated through the activation of the NADPH oxidase may play an essential role in the apoptosis induced by apigenin in HepG2 cells. These results further suggest that apigenin may be valuable for the therapeutic management of human hepatomas.