Short Report: Polymerase Chain Reaction for Chronic Trypanosoma cruzi Infection Yields Higher Sensitivity in Blood Clot Than Buffy Coat or Whole Blood Specimens
Short Report: Polymerase Chain Reaction for Chronic Trypanosoma cruzi Infection Yields Higher Sensitivity in Blood Clot Than Buffy Coat or Whole Blood Specimens
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DOI：
10.4269/ajtmh.2008.79.768
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发表时间：
2008-11-01
影响因子：
3.3
通讯作者：
Bern, Caryn
中科院分区：
文献类型：
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作者：
Fitzwater, Sean;Calderon, Maritza;Bern, Caryn
克氏锥虫聚合酶链反应（PCR）被广泛应用，但其敏感性差异很大。我们使用121/122引物对玻利维亚妇女全血、灰褐色被毛和凝块中的着丝质体微环DNA进行PCR比较。血凝块（60.1%）的敏感性明显高于黄皮毛（46.5%）或全血（40%）。血凝块的使用可以简化标本采集，同时提高灵敏度。
Trypanosoma cruzi polymerase chain reaction (PCR) is widely used, but sensitivity varies widely. We compared PCR using 121/122 primers targeting kinetoplast minicircle DNA in whole blood, buffy coat, and clot from Bolivian women. Sensitivity was significantly higher in clot (60.1 %) than buffy coat (46.5%) or whole blood (40%). The use of clot could simplify specimen collection while improving sensitivity.