Demonstration of sustained hydrogen photoproduction by immobilized, sulfur-deprived Chlamydomonas reinhardtii cells
Demonstration of sustained hydrogen photoproduction by immobilized, sulfur-deprived Chlamydomonas reinhardtii cells
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DOI：
10.1016/j.ijhydene.2005.05.002
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发表时间：
2006-04
影响因子：
7.2
通讯作者：
T. Laurinavichene;A. Fedorov;M. Ghirardi;M. Seibert;A. Tsygankov
中科院分区：
文献类型：
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作者：
T. Laurinavichene;A. Fedorov;M. Ghirardi;M. Seibert;A. Tsygankov
结果表明，固定化，硫剥夺藻类培养物可以光产生H2。在确定了最佳的材料和程序固定化的Chlamyoplasticreinhardtii在高细胞密度后，我们研究了液体混合，硫酸盐含量，乙酸水平和光照强度对H2-生产活动的文化。我们的研究结果表明：（a）液体混合是重要的，以提供均匀的条件，为固定化的文化;（B）硫剥夺是必要的固定化的文化产氢;和（c）高光强降低H2生产。在23天内，由该系统产生的最大总H2体积（160 ml反应器体积）为380 ml，并且观察到的最高H2产生速率为45 ml/天。细胞固定化显着增加的持续时间的H2-光生产阶段（长达4周），保持特定的H2光生产率类似于悬浮培养物，并表现出潜在的大量增加H2生产。
It was demonstrated that immobilized, sulfur-deprived algal cultures can photoproduce H2. After identifying the optimal material and procedures for immobilization of Chlamyodomonas reinhardtii at high cell density, we examined the effect of liquid mixing, sulfate content, acetate levels and light intensity on the H2-production activity of the culture. Our results indicate that (a) liquid mixing is important to provide homogeneous conditions for the immobilized culture; (b) sulfur deprivation is necessary for hydrogen production by immobilized cultures; and (c) high light intensity decreases H2production. The maximum total volume of H2produced by the system (160 mlof reactor volume) was 380ml over 23 days, and the highest rate of H2production observed was 45mlday-1. Cell immobilization significantly increased the duration of the H2-photoproduction phase (up to 4 weeks), maintained specific rates of H2photoproduction similar to those of suspension cultures and showed potential for large increases in H2production.